HA/Hemagglutinin Protein, H3N2 (P03437, sf9, His)

HA/Hemagglutinin Protein binds to cell surface receptors, facilitating virus attachment.It determines host range and virulence.As a class I fusion protein, it mediates fusion of virus and endosomal membranes for cell penetration.Acidic endosomes trigger HA2 conformational change, releasing fusion peptide.Multiple HA trimers are needed for fusion pore formation.HA/Hemagglutinin Protein, H3N2 (P03437, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

HA/Hemagglutinin Protein binds to sialic acid-containing receptors on the cell surface, facilitating the attachment of the virus particle to the cell. This attachment leads to the internalization of approximately two thirds of the virus particles through clathrin-dependent endocytosis and around one third through a clathrin- and caveolin-independent pathway. It plays a crucial role in determining the host range restriction and virulence. As a class I viral fusion protein, it is responsible for mediating the fusion of the endocytosed virus particle membrane with the endosomal membrane, enabling the penetration of the virus into the cell cytoplasm. The acidic environment in endosomes triggers an irreversible conformational change in HA2, resulting in the release of the fusion hydrophobic peptide. Multiple trimers of HA/Hemagglutinin Protein are necessary to form a functional fusion pore.

Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.5 μg/mL for 1%GRBC.

Virus

Sf9 insect cells

C-His

P03437 (Q17-W530)

/

Extracellular Domain

QDLPGNDNSTATLCLGHHAVPNGTLVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGIDCTLIDALLGDPHCDVFQNETWDLFVERSKAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSNACKRGPGSGFFSRLNWLTKSGSTYPVLNVTMPNNDNFDKLYIWGIHHPSTNQEQTSLYVQASGRVTVSTRRSQQTIIPNIGSRPWVRGLSSRISIYWTIVKPGDVLVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGMRNVPEKQTRGLFGAIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTRRQLRENAEEMGNGCFKIYHKCDNACIESIRNGTYDHDVYRDEALNNRFQIKGVELKSGYKDW

Approximately 66 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM Tris, pH 8.0, 150 mM NaCl, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.