HA/Hemagglutinin Protein, H3N2 (P03438, sf9, His)

The HA/Hemagglutinin Protein attaches to cell surface receptors, allowing virus attachment.It determines host range and virulence.As a class I viral fusion protein, it mediates fusion of virus and endosomal membranes for cell penetration.In acidic endosomes, HA2 undergoes irreversible conformational change, releasing fusion peptide.Multiple HA trimers are needed for fusion pore formation.HA/Hemagglutinin Protein, H3N2 (P03438, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

The HA/Hemagglutinin Protein binds to sialic acid-containing receptors on the cell surface, facilitating attachment of the virus particle. This attachment leads to internalization of about two thirds of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. It plays a crucial role in determining host range restriction and virulence. As a class I viral fusion protein, it is responsible for penetrating the cell cytoplasm by mediating the fusion of the endocytosed virus particle membrane with the endosomal membrane. In the low pH environment of endosomes, there is an irreversible conformational change in HA2, which releases the fusion hydrophobic peptide. Multiple trimers of HA are necessary for the formation of a competent fusion pore.

Virus

Sf9 insect cells

C-His

P03438 (Q17-W530)

/

Extracellular Domain

QDLPGNDNSTATLCLGHHAVPNGTLVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGIDCTLIDALLGDPHCDVFQKETWDLFVERSKAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSIACKRGPDSGFFSRLNWLTKSESTYPVLNVTMPNNDNFDKLYIWGIHHPSTNQEQTSLYVQASGRVTVSTRRSQQTIIPNIGSRPWVRGLSSRISIYWTIVKPGDVLVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGMRNVPEKQTRGLFGAIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTRRQLRENAEDMGNGCFKIYHKCDNACIESIRNGTYDHDVYRDEALNNRFQIKGVELKSGYKDW

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.