HA/Hemagglutinin Protein, H4N2 (ADN78261, sf9, His)

The hemagglutinin HA1 protein is essential for attachment of virions to host cells by binding to sialic acid-containing receptors, inducing virion internalization via clathrin-dependent or clathrin- and caveolin-independent pathways.As a class I viral fusion protein, HA1 determines host range restriction and virulence, mediating fusion between the virion membrane and the endosomal membrane.HA/Hemagglutinin Protein, H4N2 (ADN78261, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

The Hemagglutinin HA1 protein is instrumental in the attachment of virus particles to host cells by binding to sialic acid-containing receptors on the cell surface. This attachment triggers virion internalization through either clathrin-dependent endocytosis or a clathrin- and caveolin-independent pathway. HA1 plays a crucial role in determining host range restriction and virulence, functioning as a Class I viral fusion protein that facilitates the penetration of the virus into the cell cytoplasm by mediating the fusion of the endocytosed virus particle's membrane with the endosomal membrane. The low pH environment in endosomes induces an irreversible conformational change in HA2, leading to the release of the fusion hydrophobic peptide. The formation of a competent fusion pore requires the cooperative action of several trimers, underscoring the intricate mechanisms by which HA1 contributes to viral entry and infection.

Virus

Sf9 insect cells

C-His

ADN78261 (Q17-D527)

/

Partial

QNYTGNPVICMGHHAVANGTMVKTLTDDQVEVVTAQELVESQNLPELCPSPLRLVDGQTCDIINGALGSPGCDHLNGAEWDIFIERPNAVDTCYPFDVPDYQSLRSILANNGKFEFIAEEFQWSTVKQNGKSGACKRADVNDFFNRLNWLVKSDGNAYPLQNLTKVNNGDYARLYIWGVHHPSTDTEQTNLYKNNPGRVTVSTKTSQTSVVPNIGSRPWVRGQSGRISFYWTIVEPGDLIVFNTIGNLIAPRGHYKLNNQKKSTILNTATPIGSCVSKCHTDKGSLSTTKPFQNVSRIAIGDCPKYVKQGSLKLATGMRNIPEKASRGLFGAIAGFIENGWQGLIDGWYGFRHQNAEGTGTAADLKSTQAAIDQINGKLNRLIEKTNEKYHQIEKEFEQVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDVTDSEMNKLFERVRRQLRENAEDKGNGCFEIFHKCDNNCIESIRNGTYDHDVYRDEAINNRFQIQGVKLTQGYKD

Approximately 58.8 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% Glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.