1. Recombinant Proteins
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. Hemagglutinin/HA Protein, H8N4 (ABB87729, HEK293, His)

Hemagglutinin/HA Protein, H8N4 (ABB87729, HEK293, His)

Cat. No.: HY-P77034
COA Handling Instructions

The hemagglutinin HA1 protein is essential for attachment of virions to host cells by binding to sialic acid-containing receptors, inducing virion internalization via clathrin-dependent or clathrin- and caveolin-independent pathways. As a class I viral fusion protein, HA1 determines host range restriction and virulence, mediating fusion between the virion membrane and the endosomal membrane. Hemagglutinin/HA Protein, H8N4 (ABB87729, HEK293, His) is the recombinant Virus-derived Hemagglutinin/HA protein, expressed by HEK293 , with C-His labeled tag. The total length of Hemagglutinin/HA Protein, H8N4 (ABB87729, HEK293, His) is 529 a.a., with molecular weight of 70-80 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The hemagglutinin HA1 protein is essential for attachment of virions to host cells by binding to sialic acid-containing receptors, inducing virion internalization via clathrin-dependent or clathrin- and caveolin-independent pathways. As a class I viral fusion protein, HA1 determines host range restriction and virulence, mediating fusion between the virion membrane and the endosomal membrane. Hemagglutinin/HA Protein, H8N4 (ABB87729, HEK293, His) is the recombinant Virus-derived Hemagglutinin/HA protein, expressed by HEK293 , with C-His labeled tag. The total length of Hemagglutinin/HA Protein, H8N4 (ABB87729, HEK293, His) is 529 a.a., with molecular weight of 70-80 kDa.

Background

The Hemagglutinin HA1 protein is instrumental in the attachment of virus particles to host cells by binding to sialic acid-containing receptors on the cell surface. This attachment triggers virion internalization through either clathrin-dependent endocytosis or a clathrin- and caveolin-independent pathway. HA1 plays a crucial role in determining host range restriction and virulence, functioning as a Class I viral fusion protein that facilitates the penetration of the virus into the cell cytoplasm by mediating the fusion of the endocytosed virus particle's membrane with the endosomal membrane. The low pH environment in endosomes induces an irreversible conformational change in HA2, leading to the release of the fusion hydrophobic peptide. The formation of a competent fusion pore requires the cooperative action of several trimers, underscoring the intricate mechanisms by which HA1 contributes to viral entry and infection.

Species

Virus

Source

HEK293

Tag

C-His

Accession

ABB87729 (M1-K529)

Gene ID

/

Molecular Construction
N-term
H8N4 HA (M1-K529)
Accession # ABB87729
His
C-term
Synonyms
Influenza A H8N4 (A/pintail duck/Alberta/114/1979) Hemagglutinin / HA Protein (His)
Molecular Weight

70-80 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Hemagglutinin/HA Protein, H8N4 (ABB87729, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Hemagglutinin/HA Protein, H8N4 (ABB87729, HEK293, His)
Cat. No.:
HY-P77034
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