HA1/Hemagglutinin Protein, H10N3 (ACT84107, HEK293, His)

Influenza A virus is a single-stranded, negative-sense, segmented RNA virus of the family Orthomyxoviridae. Its genome harbours eight viral RNA (vRNA) segments encoding at least 17 proteins. HA functions as the receptor binding glycoprotein that binds to cell-surface sialylated glycoproteins. H10N3 avian influenza viruses (AIVs) bind to α-2, 3-linked sialic acids with high affinity and to α-2, 6-linked sialic acids with affinity^[1][2][3]. HA1/Hemagglutinin Protein, H10N3 (ACT84107, HEK293, His) is the recombinant Virus-derived HA1/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag.

Influenza A viruses are categorized into 18 different HA subtypes and 11 different NA subtypes based on the different antigenicity of their two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). Influenza viruses are prone to genetic mutations during replication, and sometimes one or two amino acid mutations can cause huge biological changes^[1].
HA functions as the receptor binding glycoprotein that binds to cell-surface sialylated glycoproteins. Particularly, influenza viruses predominantly bind to α2,3-linked sialic acid (SA) receptors abundantly located in the lower respiratory tract of birds and the intestinal tract with respect to avian influenza viruses, to α2,6-linked SA receptors. Upon virus attachment, a conformational change in the HA protein is required for the fusion reaction between the viral and endosomal membranes, after which viral ribonucleoproteins are released in the cytoplasm of the infected cell^[2].

Virus

HEK293

C-His

ACT84107 (M1-R340)

/

Partial

Approximately 44.2 kDa,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Xingtian Kong, et al. A single-amino-acid mutation at position 225 in hemagglutinin attenuates H5N6 influenza virus in mice. Emerg Microbes Infect. 2021 Dec;10(1):2052-2061.  [Content Brief]

  [2]. Khristine Kaith S Lloren, et al. Molecular Markers for Interspecies Transmission of Avian Influenza Viruses in Mammalian Hosts. Int J Mol Sci. 2017 Dec 13;18(12):2706.  [Content Brief]

  [3]. Yanna Guo, et al. Genetic and biological properties of H10N3 avian influenza viruses: A potential pandemic candidate? Transbound Emerg Dis. 2022 Sep;69(5):e3171-e3182.  [Content Brief]