1. Recombinant Proteins
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA1/Hemagglutinin Protein, H1N1 (A4U7A6, HEK293, His)

HA1/Hemagglutinin Protein, H1N1 (A4U7A6, HEK293, His)

Cat. No.: HY-P74995
Handling Instructions

The Hemagglutinin Protein (HA1/Hemagglutinin Protein) binds to receptors on cell surface, facilitating virus attachment. It triggers internalization through endocytosis. HA1/Hemagglutinin Protein determines host range and virulence, mediating fusion of virus and endosomal membrane. Acidic environment induces conformational change in HA2, releasing fusion peptide. Multiple HA1/Hemagglutinin Protein trimers form fusion pore. HA1/Hemagglutinin Protein is a homotrimer, interacting with human CACNA1C. HA1/Hemagglutinin Protein, H1N1 (A4U7A6, HEK293, His) is the recombinant Virus-derived HA1/Hemagglutinin protein, expressed by HEK293, with C-His labeled tag. The total length of HA1/Hemagglutinin Protein, H1N1 (A4U7A6, HEK293, His) is 344 a.a., with molecular weight of ~38.2 kDa.

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Description

The Hemagglutinin Protein (HA1/Hemagglutinin Protein) binds to receptors on cell surface, facilitating virus attachment. It triggers internalization through endocytosis. HA1/Hemagglutinin Protein determines host range and virulence, mediating fusion of virus and endosomal membrane. Acidic environment induces conformational change in HA2, releasing fusion peptide. Multiple HA1/Hemagglutinin Protein trimers form fusion pore. HA1/Hemagglutinin Protein is a homotrimer, interacting with human CACNA1C. HA1/Hemagglutinin Protein, H1N1 (A4U7A6, HEK293, His) is the recombinant Virus-derived HA1/Hemagglutinin protein, expressed by HEK293, with C-His labeled tag. The total length of HA1/Hemagglutinin Protein, H1N1 (A4U7A6, HEK293, His) is 344 a.a., with molecular weight of ~38.2 kDa.

Background

The Hemagglutinin Protein (HA1/Hemagglutinin Protein) binds to sialic acid-containing receptors on the cell surface, facilitating the attachment of the virus particle to the cell. This attachment triggers the internalization of the virion through clathrin-dependent endocytosis or a clathrin- and caveolin-independent pathway. HA1/Hemagglutinin Protein plays a crucial role in determining the host range restriction and virulence of the virus as a class I viral fusion protein. It is responsible for penetrating the virus into the cell cytoplasm by mediating the fusion of the endocytosed virus particle's membrane with the endosomal membrane. The acidic environment in endosomes induces an irreversible conformational change in HA2, leading to the release of the fusion hydrophobic peptide. Several trimers of HA1/Hemagglutinin Protein are required to form a competent fusion pore. HA1/Hemagglutinin Protein exists as a homotrimer, consisting of disulfide-linked HA1-HA2 subunits and interacts with human CACNA1C.

Species

Virus

Source

HEK293

Tag

C-His

Accession

A4U7A6 (M1-R344)

Gene ID

/

Molecular Construction
N-term
H1N1 HA1 (M1-R344)
Accession # A4U7A6
His
C-term
Synonyms
Influenza A H1N1 (A/Albany/12/1951) Hemagglutinin / HA1 Protein (His)
Molecular Weight

Approximately 38.2 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

HA1/Hemagglutinin Protein, H1N1 (A4U7A6, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
HA1/Hemagglutinin Protein, H1N1 (A4U7A6, HEK293, His)
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HY-P74995
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