HA1/Hemagglutinin Protein, H1N1 (A4U7A6, HEK293, His)

The hemagglutinin protein (HA1/hemagglutinin protein) binds to receptors on the cell surface and promotes virus attachment.It triggers internalization via endocytosis.HA1/Hemagglutinin Protein, H1N1 (A4U7A6, HEK293, His) is the recombinant Virus-derived HA1/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag.

The Hemagglutinin Protein (HA1/Hemagglutinin Protein) binds to sialic acid-containing receptors on the cell surface, facilitating the attachment of the virus particle to the cell. This attachment triggers the internalization of the virion through clathrin-dependent endocytosis or a clathrin- and caveolin-independent pathway. HA1/Hemagglutinin Protein plays a crucial role in determining the host range restriction and virulence of the virus as a class I viral fusion protein. It is responsible for penetrating the virus into the cell cytoplasm by mediating the fusion of the endocytosed virus particle's membrane with the endosomal membrane. The acidic environment in endosomes induces an irreversible conformational change in HA2, leading to the release of the fusion hydrophobic peptide. Several trimers of HA1/Hemagglutinin Protein are required to form a competent fusion pore. HA1/Hemagglutinin Protein exists as a homotrimer, consisting of disulfide-linked HA1-HA2 subunits and interacts with human CACNA1C.

Virus

HEK293

C-His

A4U7A6 (D18-R344)

/

Full Length of HA1 chain

DTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCRLKGIAPLQLGKCNIAGWILGNPECESLLSNRSWSYIAETPNSENGTCYPGDFADYEELREQLSSVSSFERFEIFPKERSWPKHNITRGVTAACSHAKKSSFYKNLLWLTEANGSYPNLSKSYVNNKEKEVLVLWGVHHPSNIEDQRTLYRKENAYVSVVSSNYNRRFTPEIAERPKVRGQAGRMNYYWTLLEPGDKIIFEANGNLIAPWYAFALSRGLGSGIITSNASMDECDTKCQTPQGAINSSLPFQNIHPVTIGECPKYVRSTKLRMVTGLRNIPSIQSR

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.