HA1/Hemagglutinin Protein, H3N8 (ACE81938, sf9, His)

Avian influenza virus (AIV) belongs to the genus Orthomyxovirus and contains eight single-stranded negative-sense RNA segments (PB2, PB1, PA, HA, NP, NA, M, and NS). The haemagglutinin (HA) protein presents as a homotrimer in which each monomer consists of two di-sulfide-linked HA1 and HA2 subunits after the cleavage of the HA0 precursor. The HA protein, which is responsible for receptor binding and membrane fusion, plays a key role in the influenza virus entry pathway^[1][2]. HA1/Hemagglutinin Protein, H3N8 (ACE81938, sf9, His) is the recombinant Virus-derived HA1/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

Avian influenza (AI) viruses belong to the family Orthomyxoviridae, genus Influenzavirus A, and contain a negative-sense, eight segmented RNA genome (Polymerase basic 2, PB2;Polymerase basic 1, PB1; Polymerase acid, PA; Hemagglutinin, HA; Nucleocapsid protein, NP, Neuraminidase, NA; Matrix, M; Nonstructural protein, NS). Multiple substitutions or insertions of basic amino acids in the cleavage site of the HA of H5 and H7 subtypes of LPAI viruses allow them to be cleaved by common cellular proteases, and the mutated virus can spread systemically in a host causing the highly pathogenic phenotype^[1].
Some studies have confirmed that the removal of the N-linked glycosylation sequence of HA contributes to the increase in HA affinities to an α-2,6-linked sialyl receptor and a reduction in virus growth and spread. Differences in viral shedding and the transmission of various AIV strains are dependent upon specific HA and/or NA proteins^[2].

Virus

Sf9 insect cells

C-His

ACE81938 (Q17-R344)

/

Partial

QNPISNNNTATLCLGHHAVANGTLVKTISDDQIEVTNATELVQSISMGKICNNSYRILDGRNCTLIDAMLGDPHCDVFQYENWDLFIERSSAFSNCYPYDIPDYASLRSIVASSGTLEFTAEGFTWTGVTQNGRSGACKRGSADSFFSRLNWLTKSGNSYPTLNVTMPNNKNFDKLYIWGIHHPSSNQEQTKLYIQESGRVTVSTKRSQQTIIPNIGSRPWVRGQSGRISIYWTIVKPGDILMINSNGNLVAPRGYFKLKTGKSSVMRSDVPIDICVSECITPNGSISNDKPFQNVNKVTYGKCPKYIRQNTLKLATGMRNVPEKQIR

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Sungsu Youk, et al. Rapid evolution of Mexican H7N3 highly pathogenic avian influenza viruses in poultry. PLoS One. 2019 Sep 12;14(9):e0222457.  [Content Brief]

  [2]. Lu Yao, et al. Identification of antigenic epitopes in the haemagglutinin protein of H7 avian influenza virus. Avian Pathol. 2020 Feb;49(1):62-73.  [Content Brief]