1. Recombinant Proteins
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA1/Hemagglutinin Protein, H7N3 (CAF33017, HEK293, His)

HA1/Hemagglutinin Protein, H7N3 (CAF33017, HEK293, His)

Cat. No.: HY-P75884
Handling Instructions

Avian influenza virus (AIV) belongs to the genus Orthomyxovirus and contains eight single-stranded negative-sense RNA segments (PB2, PB1, PA, HA, NP, NA, M, and NS). The haemagglutinin (HA) protein presents as a homotrimer in which each monomer consists of two di-sulfide-linked HA1 and HA2 subunits after the cleavage of the HA0 precursor. The HA protein, which is responsible for receptor binding and membrane fusion, plays a key role in the influenza virus entry pathway. HA1/Hemagglutinin Protein, H7N3 (CAF33017, HEK293, His) is the recombinant Virus-derived HA1/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag. The total length of HA1/Hemagglutinin Protein, H7N3 (CAF33017, HEK293, His) is 349 a.a., with molecular weight of ~47.3 kDa.

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Description

Avian influenza virus (AIV) belongs to the genus Orthomyxovirus and contains eight single-stranded negative-sense RNA segments (PB2, PB1, PA, HA, NP, NA, M, and NS). The haemagglutinin (HA) protein presents as a homotrimer in which each monomer consists of two di-sulfide-linked HA1 and HA2 subunits after the cleavage of the HA0 precursor. The HA protein, which is responsible for receptor binding and membrane fusion, plays a key role in the influenza virus entry pathway[1][2]. HA1/Hemagglutinin Protein, H7N3 (CAF33017, HEK293, His) is the recombinant Virus-derived HA1/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag. The total length of HA1/Hemagglutinin Protein, H7N3 (CAF33017, HEK293, His) is 349 a.a., with molecular weight of ~47.3 kDa.

Background

Avian influenza (AI) viruses belong to the family Orthomyxoviridae, genus Influenzavirus A, and contain a negative-sense, eight segmented RNA genome (Polymerase basic 2, PB2;Polymerase basic 1, PB1; Polymerase acid, PA; Hemagglutinin, HA; Nucleocapsid protein, NP, Neuraminidase, NA; Matrix, M; Nonstructural protein, NS). Multiple substitutions or insertions of basic amino acids in the cleavage site of the HA of H5 and H7 subtypes of LPAI viruses allow them to be cleaved by common cellular proteases, and the mutated virus can spread systemically in a host causing the highly pathogenic phenotype[1].
Some studies have confirmed that the removal of the N-linked glycosylation sequence of HA contributes to the increase in HA affinities to an α-2,6-linked sialyl receptor and a reduction in virus growth and spread. Differences in viral shedding and the transmission of various AIV strains are dependent upon specific HA and/or NA proteins[2].

Species

Virus

Source

HEK293

Tag

C-His

Accession

CAF33017 (M1-R349)

Gene ID

/

Molecular Construction
N-term
H7N3 HA1 (M1-R349)
Accession # CAF33017
His
C-term
Synonyms
Influenza A H7N3 (A/turkey/Italy/214845/2002) Hemagglutinin Protein (HA1 Subunit) (His)
Molecular Weight

Approximately 47.3 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

HA1/Hemagglutinin Protein, H7N3 (CAF33017, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
HA1/Hemagglutinin Protein, H7N3 (CAF33017, HEK293, His)
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HY-P75884
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