1. Recombinant Proteins
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA1/Hemagglutinin Protein, Influenza B (EPI529345, HEK293, His)

HA1/Hemagglutinin Protein, Influenza B (EPI529345, HEK293, His)

Cat. No.: HY-P74906
Handling Instructions

HA/Hemagglutinin proteins are Class I fusion proteins with multiple functions as attachment factors and membrane fusion proteins. HA/Hemagglutinin proteins can bind to cell surface receptors containing sialic acid, promote the attachment of virus particles to host cells, and play an important role in viral host range limitation and virulence. HA1/Hemagglutinin Protein, Influenza B (EPI529345, HEK293, His) is the recombinant Virus-derived HA1/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag. The total length of HA1/Hemagglutinin Protein, Influenza B (EPI529345, HEK293, His) is 361 a.a., with molecular weight of ~39.1 kDa.

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Description

HA/Hemagglutinin proteins are Class I fusion proteins with multiple functions as attachment factors and membrane fusion proteins. HA/Hemagglutinin proteins can bind to cell surface receptors containing sialic acid, promote the attachment of virus particles to host cells, and play an important role in viral host range limitation and virulence. HA1/Hemagglutinin Protein, Influenza B (EPI529345, HEK293, His) is the recombinant Virus-derived HA1/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag. The total length of HA1/Hemagglutinin Protein, Influenza B (EPI529345, HEK293, His) is 361 a.a., with molecular weight of ~39.1 kDa.

Background

Hemagglutinin is a Class I fusion protein, which has multiple functions as an attachment factor and membrane fusion protein, and plays a crucial role in the viral life cycle. It binds to cell surface receptors containing sialic acid, promoting the attachment of viral particles to host cells. This attachment leads to internalization of virions through clathrin-dependent endocytosis pathways or pathways independent of clathrin and poretin. Hyaluronic acid protein is a kind of viral fusion protein, which is responsible for mediating the fusion of endocytotic virus particle membrane and endosomal membrane. This fusion event allows the virus to enter the cytoplasm of the cell. The low pH environment of the endosome triggers irreversible conformational changes in the protein HA2 subunit, resulting in the release of fusion hydrophobic peptides. Multiple trimers of HA proteins are necessary to form a qualified fusion pore. The function of hyaluronic acid protein in receptor binding, viral internalization, fusion, and membrane penetration is critical in determining viral host range limitation and virulence. Influenza hemagglutinin (HA) is a homologous trimer glycoprotein found on the surface of influenza virus and is an integral part of its infectivity[1][2][3].

Species

Virus

Source

HEK293

Tag

C-His

Accession

EPI529345 (M1-R361)

Gene ID

/

Molecular Construction
N-term
HA1 (M1-R361)
Accession # EPI529345
His
C-term
Synonyms
Influenza B (B/PHUKET/3073/2013) Hemagglutinin / HA1 Protein (HEK293, His)
Molecular Weight

Approximately 39.1 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

HA1/Hemagglutinin Protein, Influenza B (EPI529345, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HA1/Hemagglutinin Protein, Influenza B (EPI529345, HEK293, His)
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HY-P74906
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