Heparan Sulfate-6-O-Sulfotransferase-3 Protein, Mouse (HEK293, His)

Heparan Sulfate-6-O-Sulfotransferase-3 (HS6ST3) is an enzyme involved in modifying heparan sulfate proteoglycan biosynthesis. It is predicted to be a membrane-bound integral component. HS6ST3 shows specific expression in the adult cortex (RPKM 1.3) and frontal lobe (RPKM 0.8), implying its potential role in determining the sulfation patterns of heparan sulfate in these contexts. Heparan Sulfate-6-O-Sulfotransferase-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Heparan Sulfate-6-O-Sulfotransferase-3 protein, expressed by HEK293 , with N-His labeled tag.

Heparan Sulfate-6-O-Sulfotransferase-3 (HS6ST3) is a protein that exhibits heparan sulfate 6-O-sulfotransferase activity and is implicated in the enzymatic modification of heparan sulfate proteoglycan biosynthetic processes. Predicted to be situated in the membrane as an integral component, HS6ST3 demonstrates biased expression in various tissues, including the cortex adult (RPKM 1.3) and frontal lobe adult (RPKM 0.8), suggesting its potential role in shaping the sulfation patterns of heparan sulfate within these contexts.

Measured by its ability to transfer sulfate from PAPS to heparan sulfate. The specific activity is 73.892 pmol/min/μg that incubate at 37 ºC for 20 minutes.

Mouse

HEK293

N-6*His

Q9QYK4/NP_056635 (P28-W470)

50787  [NCBI]

Lumenal Domain

PSCTSSCTNFGEQLRSGEARPPAVPSPARRAQAPLDEWERRPQLPPPPRGPPEGSRGVAAPEDEDEDPGDPEEEEEEEEEEPDPEAPENGSLPRFVPRFNFTLKDLTRFVDFNIKGRDVIVFLHIQKTGGTTFGRHLVKNIRLEQPCSCKAGQKKCTCHRPGKKETWLFSRFSTGWSCGLHADWTELTNCVPAIMEKKDCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTPDELPTCYPGDDWSGVSLREFMDCSYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVDINDGARQHIEELNFLDMQLYEYAKDLFQQRYHHTKQLEHQRDRQKRREERRLQREHRAHRWPKEDRAMEGTVTEDYNSQVVRW

Approximately 75-110 kDa due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.