Heparinase III Protein, P. heparinus (His)

Heparinase III Protein is the unique member of the heparinase family of heparin-degrading lyases that recognizes the ubiquitous cell-surface heparan sulfate proteoglycans as its primary substrate. Heparinase III functions in cleaving metazoan heparan sulfate and providing carbon, nitrogen and sulfate sources for microorganisms. Furthermore, Heparinase III is cardioprotective in a dose-dependent manner, preserves endothelial function and attenuates PMN adherence to the coronary vascular endothelium. Heparinase III Protein, P. heparinus (His) is the recombinant Heparinase III protein, expressed by E. coli , with N-6*His labeled tag.

Heparinase III is the unique member of the heparinase family of heparin-degrading lyases that recognizes the ubiquitous cell-surface heparan sulfate proteoglycans as its primary substrate. Heparinase III functions in cleaving metazoan heparan sulfate and providing carbon, nitrogen and sulfate sources for microorganisms. Heparinase III specifically cleaves heparan sulfate-rich regions of acidic polysaccharides. It acts primarily at the glycosidic linkage between N-sulfated or N-acetylated glucosamine (GlcNS or GlcNAc) and glucuronic acid (GlcA) as primary sites on the condition that the C2 GlcA is not sulfated. It can limit proliferation by affecting heparan sulfate proteoglycan binding growth factors following arterial injury. Furthermore, Heparinase III is cardioprotective in a dose-dependent manner, preserves endothelial function and attenuates PMN adherence to the coronary vascular endothelium^[1][2][3][4].

Measured by its ability to liberate oligosaccharides from heparin. Which can be measured by absorbance at 232 nm. The specific activity is 432.279935 pmol/min/µg, as measured under the described conditions.

Others

E. coli

N-6*His

AAB18278 (Q25-P659)

8254931

Full Length of Mature Protein

QSSSITRKDFDHINLEYSGLEKVNKAVAAGNYDDAAKALLAYYREKSKAREPDFSNAEKPADIRQPIDKVTREMADKALVHQFQPHKGYGYFDYGKDINWQMWPVKDNEVRWQLHRVKWWQAMALVYHATGDEKYAREWVYQYSDWARKNPLGLSQDNDKFVWRPLEVSDRVQSLPPTFSLFVNSPAFTPAFLMEFLNSYHQQADYLSTHYAEQGNHRLFEAQRNLFAGVSFPEFKDSPRWRQTGISVLNTEIKKQVYADGMQFELSPIYHVAAIDIFLKAYGSAKRVNLEKEFPQSYVQTVENMIMALISISLPDYNTPMFGDSWITDKNFRMAQFASWARVFPANQAIKYFATDGKQGKAPNFLSKALSNAGFYTFRSGWDKNATVMVLKASPPGEFHAQPDNGTFELFIKGRNFTPDAGVFVYSGDEAIMKLRNWYRQTRIHSTLTLDNQNMVITKARQNKWETGNNLDVLTYTNPSYPNLDHQRSVLFINKKYFLVIDRAIGEATGNLGVHWQLKEDSNPVFDKTKNRVYTTYRDGNNLMIQSLNADRTSLNEEEGKVSYVYNKELKRPAFVFEKPKKNAGTQNFVSIVYPYDGQKAPEISIRENKGNDFEKGKLNLTLTINGKQQLVLVP

Approximately 74 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 300 mM NaCl, pH 7.4, 20% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. /biology-dictionary/kb-cell.html  [Content Brief]