Hyaluronidase-1/HYAL1 Protein, Human (HEK293, His, solution)

Hyaluronidase-1/HYAL1 Protein is implicated in tumor progression, suggesting its role in cancer advancement. It also inhibits TGFB1-enhanced cell growth, revealing its regulatory function. HYAL1's dual impact on promoting tumor progression and inhibiting specific cellular processes underscores its complex involvement in cellular behavior and potential significance in tumorigenesis. Hyaluronidase-1/HYAL1 Protein, Human (HEK293, His, solution) is the recombinant human-derived Hyaluronidase-1/HYAL1 protein, expressed by HEK293 , with C-6*His labeled tag.

The Hyaluronidase-1/HYAL1 protein emerges as a potential contributor to tumor progression, suggesting its involvement in the intricate mechanisms that facilitate cancer advancement. Additionally, it exhibits the ability to impede TGFB1-enhanced cell growth, underscoring its regulatory function in restraining specific cellular processes. The dual nature of its impact on both promoting tumor progression and inhibiting TGFB1-enhanced cell growth underscores the complexity of HYAL1's involvement in cellular behavior and highlights its potential significance in the context of tumorigenesis.

Measured in a cell proliferation assay using DU145 cells. The ED₅₀ for this effect is ≤1 ng/mL, corresponding to a specific activity is ≥1×10⁶ units/mg.

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  Measured in a cell proliferation assay using DU145 cells. The ED₅₀ for this effect is 0.912 ng/mL, corresponding to a specific activity is 1.1×10⁶ units/mg.

Human

HEK293

C-6*His

Q12794-1 (F22-W435)

3373  [NCBI]

Full Length of Isoform-1 Mature Protein

FRGPLLPNRPFTTVWNANTQWCLERHGVDVDVSVFDVVANPGQTFRGPDMTIFYSSQLGTYPYYTPTGEPVFGGLPQNASLIAHLARTFQDILAAIPAPDFSGLAVIDWEAWRPRWAFNWDTKDIYRQRSRALVQAQHPDWPAPQVEAVAQDQFQGAARAWMAGTLQLGRALRPRGLWGFYGFPDCYNYDFLSPNYTGQCPSGIRAQNDQLGWLWGQSRALYPSIYMPAVLEGTGKSQMYVQHRVAEAFRVAVAAGDPNLPVLPYVQIFYDTTNHFLPLDELEHSLGESAAQGAAGVVLWVSWENTRTKESCQAIKEYMDTTLGPFILNVTSGALLCSQALCSGHGRCVRRTSHPKALLLLNPASFSIQLTPGGGPLSLRGALSLEDQAQMAVEFKCRCYPGWQAPWCERKSMW

Approximately 50-65 kDa due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.