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  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-alpha
  5. Interferon alpha-B
  6. IFN-alpha B/IFNA8 Protein, Cynomolgus (HEK293, mFc, solution)

IFN-alpha B/IFNA8 Protein, Cynomolgus (HEK293, mFc, solution)

Cat. No.: HY-P73249
Handling Instructions Technical Support

IFN-alpha B/IFNA8 protein is a member of the alpha/beta interferon family. IFN-alpha B/IFNA8 Protein, Cynomolgus (HEK293, mFc, solution) is the recombinant cynomolgus-derived IFN-alpha B/IFNA8 protein, expressed by HEK293 , with C-mFc labeled tag.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

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Description

IFN-alpha B/IFNA8 protein is a member of the alpha/beta interferon family. IFN-alpha B/IFNA8 Protein, Cynomolgus (HEK293, mFc, solution) is the recombinant cynomolgus-derived IFN-alpha B/IFNA8 protein, expressed by HEK293 , with C-mFc labeled tag.

Background

IFN-alpha B/IFNA8 protein belongs to the alpha/beta interferon family.

Biological Activity

Measured in antiviral assay using WISH human amnion cells infected with vesicular stomatitisvirus (VSV).The ED50 for this effect is typically 0.1-0.6 ng/mL.

Species

Cynomolgus

Source

HEK293

Tag

C-mFc

Accession

G7PS89 (C24-E189)

Gene ID
Molecular Construction
N-term
IFNA8 (C24-E189)
Accession # G7PS89
mFc
C-term
Protein Length

Full Length of Mature Protein

Synonyms
IFNA8; IFN-alpha 8; Interferon alpha-B; Interferon alpha-B2; LeIF B
AA Sequence

CDLPQTHSLGYRRPLVLLAQMRRISPFSCLKDRHDFELPQEEFDDKNFQKAQAISVLHEIIQQTFNLFNTKNSSAAFNETLLDEFYIELDQQLNDLESCVMQEVGVTETHLMYEDSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSFSLSINLQKRLKSKE

Predicted Molecular Mass
46 kDa
Molecular Weight

Approximately 55-65 kDa, based on SDS-PAGE under reducing conditions.

Structure/Form
Disulfide-linked homodimer
Purity
  • ≥ 95%, as determined by reducing SDS-PAGE.
Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References

IFN-alpha B/IFNA8 Protein, Cynomolgus (HEK293, mFc, solution) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IFN-alpha B/IFNA8 Protein, Cynomolgus (HEK293, mFc, solution)
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HY-P73249
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