1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. IGF family
  4. IGFBP-5
  5. IGFBP-5 Protein, Canine (HEK293, Fc)

IGFBP-5 Protein, Canine (HEK293, Fc)

Cat. No.: HY-P76410
Handling Instructions

IGFBP-5 is only one of a family of six IGFBPs. IGFBP-5 is a 29 kDa phosphorylated and glycosylated protein, which is secreted by a wide variety of cell types during different growth conditions. IGFBP-5, the most conserved IGFBP, is the major protein secreted by skeletal muscles. IGFBP-5 Protein, Canine (HEK293, Fc) is the recombinant canine-derived IGFBP-5 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of IGFBP-5 Protein, Canine (HEK293, Fc) is 252 a.a., with molecular weight of ~40 & 43 & 45 & 65 KDa, respectively.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

IGFBP-5 is only one of a family of six IGFBPs. IGFBP-5 is a 29 kDa phosphorylated and glycosylated protein, which is secreted by a wide variety of cell types during different growth conditions. IGFBP-5, the most conserved IGFBP, is the major protein secreted by skeletal muscles. IGFBP-5 Protein, Canine (HEK293, Fc) is the recombinant canine-derived IGFBP-5 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of IGFBP-5 Protein, Canine (HEK293, Fc) is 252 a.a., with molecular weight of ~40 & 43 & 45 & 65 KDa, respectively.

Background

IGFBP-5 is only one of a family of six IGFBPs. IGFBP-5 is a 29 kDa phosphorylated and glycosylated protein, which is secreted by a wide variety of cell types during different growth conditions. IGFBP-5, the most conserved IGFBP, is the major protein secreted by skeletal muscles. IGFBP-5 enables fibronectin binding and is involved in regulation of insulin-like growth factor receptor signaling pathway[1][2][3][4].

Biological Activity

Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED50 this effect is 1.468 µg/mL, corresponding to a specific activity is 681.199 U/mg.

Species

Canine

Source

HEK293

Tag

C-hFc

Accession

F1PEQ5 (L20-E271)

Gene ID

610316  [NCBI]

Molecular Construction
N-term
IGFBP-5 (L20-E271)
Accession # F1PEQ5
hFc
C-term
Synonyms
Insulin-like growth factor-binding protein 5; IGFBP5
AA Sequence

LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQAKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE

Molecular Weight

Approximately 40&43&45&65 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IGFBP-5 Protein, Canine (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IGFBP-5 Protein, Canine (HEK293, Fc)
Cat. No.:
HY-P76410
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