IgG4 Fc Protein, Human (Biotinylated, HEK293, Avi)

Measured by its binding ability in a functional ELISA.Immobilized Protein A at 1 μg/mL (100 μL/well) can bind Biotinylated Human IgG4 Fc Protein, Avitag.

Human

HEK293

C-Avi

P01861-1 (E99-L327)

3503

Partial

Biotin

Approximately 35-38 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with 10% trehalose as protectant.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.