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IIdD Protein, E.coli (His)

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GLO1/Glyoxalase I Protein catalyzes the conversion of hemimercaptal to S-lactoylglutathione, detoxifying cytotoxic methylglyoxal produced in glycolysis. Beyond detoxification, GLO1 regulates NF-kappa-B transcriptional activity in TNF-induced pathways, indicating its role in inflammation. Additionally, GLO1 is crucial for normal osteoclastogenesis, emphasizing its broader involvement in cellular processes. IIdD Protein, E.coli (His) is the recombinant E. coli-derived IIdD protein, expressed by E. coli , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: E.coli
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • IIdD Protein, E.coli (His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

GLO1/Glyoxalase I Protein catalyzes the conversion of hemimercaptal to S-lactoylglutathione, detoxifying cytotoxic methylglyoxal produced in glycolysis. Beyond detoxification, GLO1 regulates NF-kappa-B transcriptional activity in TNF-induced pathways, indicating its role in inflammation. Additionally, GLO1 is crucial for normal osteoclastogenesis, emphasizing its broader involvement in cellular processes. IIdD Protein, E.coli (His) is the recombinant E. coli-derived IIdD protein, expressed by E. coli , with N-His labeled tag.

Background

GLO1/Glyoxalase I protein serves as a catalyst in the conversion of hemimercaptal, generated from the reaction between methylglyoxal and glutathione, into S-lactoylglutathione. This enzymatic activity plays a crucial role in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis. Additionally, GLO1 is implicated in the regulation of TNF-induced transcriptional activity of NF-kappa-B, suggesting its involvement in inflammatory signaling pathways. Furthermore, GLO1 is essential for normal osteoclastogenesis, highlighting its significance in cellular processes beyond its role in detoxification.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species E.coli
  • Source E. coli
  • Tag N-His
  • Accession

    A8A670 (M1-A396)

  • Gene ID
    /
  • Molecular Construction
    • N-term
    • His
    • IIdD (M1-A396)
      Accession # A8A67
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    lldD; EcHS_A3817L-lactate dehydrogenase

  • AA Sequence

    MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSEVALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIPFTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPTPGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLEDYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLYALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAKGNAA

  • Predicted Molecular Mass

    46.7 kDa

  • Molecular Weight

    Approximately 44 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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