IL-13R alpha 1 Protein, Cynomolgus (HEK293, His)

The IL-13R α 1 protein binds to IL13 with low affinity and, when combined with IL4RA, forms a functional IL13 receptor. It also functions together with IL4RA as an alternative accessory protein for IL4 signaling. IL-13R alpha 1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IL-13R alpha 1 protein, expressed by HEK293 , with C-His labeled tag.

The IL-13R alpha 1 protein exhibits a low-affinity binding to interleukin-13 (IL13) and, when partnered with IL4RA, forms a functional receptor for IL13. Furthermore, IL-13R alpha 1 serves as an alternate accessory protein alongside IL4RA for interleukin-4 (IL4) signaling. Although it cannot replace the function of IL2RG in enabling enhanced interleukin-2 (IL2) binding activity, IL-13R alpha 1 plays a crucial role in mediating IL4 and IL13 responses. The interleukin-13 receptor complex comprises IL4R, IL13RA1, and potentially other components, illustrating the intricacies of its molecular architecture. Additionally, IL-13R alpha 1 interacts with TRAF3IP1 and IL4, contributing to its involvement in diverse signaling pathways and underscoring its significance as a component of the interleukin-13 receptor system.

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus IL-13R alpha 1 at 4 µg/mL (100 µL/well) can bind Recombinant human IL-13. The ED₅₀ for this effect is 42.49 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus IL-13R alpha 1 at 4 µg/mL (100 µL/well) can bind Recombinant human IL-13 .The ED₅₀ for this effect is 42.49 ng/mL.

Cynomolgus

HEK293

C-6*His

XP_005594500 (A25-T341)

102135058  [NCBI]

Partial

APTETQPPVTNLSVSVENLCTVIWTWNPPEGASPNCSLWYFSHFGDKQDKKLAPETRRSKEVPLNEKICLQVGSQCSTNESEKPSILVEKCISPPEGDPESAVTELQCIWHNLSYMQCSWLPGRNTSPDTNYTLYYWHRSLEKIRQCEEIYKEGQYFGCSFDLTKVKDSSFEQHSVQIMVKDYAGKIKPSFNIVPLTSRVKPDPPHIKNLSFHNGDLHVQWENPQNFISRCLFYEVEVNNSQTETHNVFSVQEAKCQNPEFERNVENTSCFMVPGVLPDTLNTVRIRVKTNKLCYEDDKLWSNWSQEMSIGKKRNST

Approximately 50-70 kDa due to the glycosylation

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.