1. Recombinant Proteins
  2. Cytokines and Growth Factors CD Antigens Receptor Proteins
  3. Interleukin & Receptors NK Cell CD Proteins Cytokine Receptors
  4. IL-2 Receptor IL-2R beta/CD122
  5. IL2RB-IL2RA-IL2RG Protein, Human (HEK293, His, Twin Strep)

IL2RB-IL2RA-IL2RG Protein, Human (HEK293, His, Twin Strep)

Cat. No.: HY-P704708
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Tamaño Precio Stock Cantidad
100 μg Obtener un presupuesto 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
> 100 μg   Obtener un presupuesto  
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  • Actividad biológica

  • Technical Parameters

  • Properties

  • Descripciòn

Actividad biológica

Measured by its binding ability in a functional ELISA. Immobilized Human IL-2, Tag Free at 5 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RA&IL-2RG, His Tag&Twin Strep Tag with a linear range of 0.01-0.313 μg/mL.

Species

Human

Source

HEK293

Tag

C-Twin-Strep;C-His

Accession

P14784 (A27-D239)&P01589 (G22-S212)&P31785-1 (L23-N254)

Gene ID

3560&3559&3561

Synonyms
IL-2R beta & IL-2R alpha & IL-2R gamma
Predicted Molecular Mass
29.9 kDa (IL-2RB) & 55.8 kDa (IL-2RA&IL-2RG).
Peso molecular

Approximately 37 kDa,42 kDa and 90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Glycosylation
Yes
Pureza

≥ 90%, as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from 0.22 μm filtered solution of PBS, pH 7.4 with 10% trehalose as protectant.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Envío

Room temperature in continental US; may vary elsewhere.

Documentación
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL2RB-IL2RA-IL2RG Protein, Human (HEK293, His, Twin Strep)

 

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Nombre del producto:
IL2RB-IL2RA-IL2RG Protein, Human (HEK293, His, Twin Strep)
Cat. No.:
HY-P704708
Cantidad:
MCE Japan Authorized Agent: