IL2RB-IL2RG Heterodimer Protein, Mouse (Biotinylated, HEK293, Fc-Avi)

Measured by its binding ability in a functional ELISA. Immobilized Mouse IL-2, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-2RB&IL-2RG Protein, Fc,Avitag with a linear range of ≤ 1.0 μg/mL.

Mouse

HEK293

C-Avi;C-hFc

P16297 (A27-E240)&P34902 (W23-A263)

16185&16186

Biotin

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

Approximately 65-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

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It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.