ITPase Protein, Human (His)

ITPase protein is a pyrophosphatase that is essential in nucleotide metabolism and can hydrolyze non-classical purine nucleotides such as ITP, dITP, dHAPTP and XTP into their respective monophosphate derivatives. It lacks distinction between deoxy and ribose forms. ITPase Protein, Human (His) is the recombinant human-derived ITPase protein, expressed by E. coli , with C-6*His labeled tag. The total length of ITPase Protein, Human (His) is 193 a.a., with molecular weight of ~21.0 kDa.

ITPase, a pyrophosphatase, plays a pivotal role in nucleotide metabolism by hydrolyzing non-canonical purine nucleotides, including inosine triphosphate (ITP), deoxyinosine triphosphate (dITP), 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP), and xanthosine 5'-triphosphate (XTP), into their respective monophosphate derivatives. This enzyme demonstrates a lack of discrimination between deoxy- and ribose forms. The primary function of ITPase is presumed to be the exclusion of non-canonical purines from RNA and DNA precursor pools. By preventing the incorporation of these purines into RNA and DNA, ITPase safeguards the integrity of genetic material, contributing to the maintenance of genomic stability and averting potential chromosomal lesions.

Human

E. coli

C-6*His

Q9BY32 (A2-A194)

3704  [NCBI]

AASLVGKKIVFVTGNAKKLEEVVQILGDKFPCTLVAQKIDLPEYQGEPDEISIQKCQEAVRQVQGPVLVEDTCLCFNALGGLPGPYIKWFLEKLKPEGLHQLLAGFEDKSAYALCTFALSTGDPSQPVRLFRGRTSGRIVAPRGCQDFGWDPCFQPDGYEQTYAEMPKAEKNAVSHRFRALLELQEYFGSLAA

Approximately 21.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.