Kallikrein-6 Protein, Human (HEK293, His)

Kallikrein-6 is a serine protease that exhibits substrate preference and activity against different substrates. It degrades alpha-synuclein, plays a role in synucleinopathies, and may regulate axonal growth after spinal cord injury. Kallikrein-6 Protein, Human (HEK293, His) is the recombinant human-derived Kallikrein-6 protein, expressed by HEK 293 , with C-6*His labeled tag.

Kallikrein-6, a serine protease, displays a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. It exhibits activity against diverse substrates, including amyloid precursor protein, myelin basic protein, gelatin, casein, and extracellular matrix proteins such as fibronectin, laminin, vitronectin, and collagen. Notably, Kallikrein-6 degrades alpha-synuclein, preventing its polymerization and suggesting a potential involvement in the pathogenesis of Parkinson's disease and other synucleinopathies. Additionally, it may play a role in regulating axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody demonstrate reduced migration, indicating a potential role in invasion and metastasis. Kallikrein-6 is susceptible to inhibition by various serine protease inhibitors, including soybean trypsin inhibitor, benzamidine, and serpins. Furthermore, its activity is modulated by several glycosaminoglycans, such as chondroitin sulfate, dermatan sulfate, heparan sulfate, and heparin.

Measured by its ability to cleave 100 µM fluorogenic peptide substrate Boc-QAR-AMC (HY-134432B) that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is >1300 pmol/min/μg. (Activation description: The proenzyme needs to be activated by Lysyl-Endopeptidase for an activated form.)

Human

HEK293

C-6*His

Q92876-1 (E17-K244)

5653  [NCBI]

Full Length of Isoform-1 Mature Protein

EEQNKLVHGGPCDKTSHPYQAALYTSGHLLCGGVLIHPLWVLTAAHCKKPNLQVFLGKHNLRQRESSQEQSSVVRAVIHPDYDAASHDQDIMLLRLARPAKLSELIQPLPLERDCSANTTSCHILGWGKTADGDFPDTIQCAYIHLVSREECEHAYPGQITQNMLCAGDEKYGKDSCQGDSGGPLVCGDHLRGLVSWGNIPCGSKEKPGVYTNVCRYTNWIQKTIQAK

Approximately 32 kDa & 21-24 kDa & 10 kDa, based on SDS-PAGE under reducing conditions, due to autolysis.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 5 mM HCl, 150 mM NaCl.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.