Ketohexokinase/KHK Protein, Human (HEK293, His)

Ketohexokinase (KHK) proteins play a key role in carbohydrate metabolism by catalyzing the phosphorylation of ketoglycosides into fructose-1-phosphate. This enzyme activity is a key step in the utilization and processing of fructose within cells. Ketohexokinase/KHK Protein, Human (HEK293, His) is the recombinant human-derived Ketohexokinase/KHK protein, expressed by HEK293 , with C-6*His labeled tag.

The Ketohexokinase (KHK) protein is a crucial enzyme that catalyzes the phosphorylation of the ketose sugar fructose, converting it to fructose-1-phosphate. This phosphorylation reaction represents a key step in fructose metabolism and is pivotal for regulating various cellular processes. By initiating the conversion of fructose to fructose-1-phosphate, KHK plays a central role in diverting fructose away from glycolysis and towards alternative metabolic pathways. This enzyme is particularly important in the liver, where it contributes to the regulation of fructose metabolism and its potential impact on lipogenesis and triglyceride synthesis. Understanding the functions of KHK provides insights into the molecular mechanisms governing fructose utilization and its implications for metabolic homeostasis.

Biological activity has been tested in vitro.

Human

HEK293

C-6*His

AAH06233.1 (M1-V298)

3795  [NCBI]

Full Length

MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSMAPGHVADFLVADFRRRGVDVSQVAWQSKGDTPSSCCIINNSNGNRTIVLHDTSLPDVSATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRGLYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRFGCQVAGKKCGLQGFDGIV

Approximately 30.0 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 50nM KCl, 10% glycerol, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.