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KIRREL3/NEPH2 Protein, Rat (HEK293, His)

Cat. No.: HY-P77046
SDS COA Handling Instructions

The KIRREL3/NEPH2 protein plays a critical role in the formation of precise synapses, particularly in hippocampal mossy fiber synapses, where it is responsible for coordinating mossy fiber filopodia. It acts in a homologous manner to stabilize filopodia contacts and facilitate subsequent synapse formation. KIRREL3/NEPH2 Protein, Rat (HEK293, His) is the recombinant rat-derived KIRREL3/NEPH2 protein, expressed by HEK293 , with C-His labeled tag. The total length of KIRREL3/NEPH2 Protein, Rat (HEK293, His) is 502 a.a., with molecular weight of 60-64 kDa.

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Description

The KIRREL3/NEPH2 protein plays a critical role in the formation of precise synapses, particularly in hippocampal mossy fiber synapses, where it is responsible for coordinating mossy fiber filopodia. It acts in a homologous manner to stabilize filopodia contacts and facilitate subsequent synapse formation. KIRREL3/NEPH2 Protein, Rat (HEK293, His) is the recombinant rat-derived KIRREL3/NEPH2 protein, expressed by HEK293 , with C-His labeled tag. The total length of KIRREL3/NEPH2 Protein, Rat (HEK293, His) is 502 a.a., with molecular weight of 60-64 kDa.

Background

The KIRREL3/NEPH2 protein emerges as a pivotal synaptic adhesion molecule essential for the precise formation of target-specific synapses, particularly notable at hippocampal mossy fiber synapses. Its significance extends to the orchestration of mossy fiber filopodia, critical synaptic structures linking dentate granule and GABA neurons. Functionally, KIRREL3/NEPH2 likely serves as a homophilic adhesion molecule, fostering trans-cellular interactions and playing a key role in stabilizing mossy fiber filipodia contacts, thereby facilitating subsequent synapse formation. Beyond the hippocampus, KIRREL3/NEPH2 is vital for the coalescence of vomeronasal sensory neuron axons. Moreover, it may play a role in the hematopoietic supportive capacity of stroma cells, with its secreted extracellular domain directly supporting hematopoietic stem cells. Functioning as a homodimer, KIRREL3/NEPH2 mediates homophilic interactions crucial for promoting cell adhesion. Additionally, it engages in a spectrum of protein-protein interactions, including the formation of heterodimers with NPHS1, interactions with NPHS2/podocin, CASK, MAP1B, MYO16, ATP1B1, SHMT2, and UFC1, underscoring its multifaceted and integral role in diverse cellular processes.

Species

Rat

Source

HEK293

Tag

C-His

Accession

Q09GS6 (L22-A523)

Gene ID
Molecular Construction
N-term
KIRREL3 (L22-A523)
Accession # Q09GS6
His
C-term
Synonyms
Kin of IRRE-like protein 3; Nephrin-like protein 2; Kiaa1867; Neph2
AA Sequence

LQRRGCCLVLGYMAKDKFRRMNEGQVYSFSQQPQDQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRSRPARLTVLVPPDDPIILGGPVISLRAGDPLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESIVSTLFISPGDVENGQSIVCRATNKAIPGGKETSVTIDIQHPPLVNLSVEPQPVLEDNIVTFHCSAKANPAVTQYRWAKRGHIIKEASGELYRTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTSEPQSLLVDLGSDAVFSCAWIGNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETVNTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQESVPMA

Molecular Weight

Approximately 60-80 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

KIRREL3/NEPH2 Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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KIRREL3/NEPH2 Protein, Rat (HEK293, His)
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HY-P77046
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