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  3. Large envelope Protein, HBV-C (P.pastoris, 180a.a, His)

Large envelope Protein, HBV-C (P.pastoris, 180a.a, His)

Cat. No.: HY-P702559
Handling Instructions

The large envelope protein is crucial in its two conformations, "outside" (Le-HBsAg) and "inside" (Li-HBsAg). Externally, it binds the virus to cellular receptors, initiates infection, determines species specificity, and promotes viral particle internalization via caveolin-mediated endocytosis. Large envelope Protein, HBV-C (P.pastoris, 180a.a, His) is the recombinant Virus-derived Large envelope protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Large envelope Protein, HBV-C (P.pastoris, 180a.a, His) is 180 a.a., with molecular weight of 20.9 kDa.

For research use only. We do not sell to patients.

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Description

The large envelope protein is crucial in its two conformations, "outside" (Le-HBsAg) and "inside" (Li-HBsAg). Externally, it binds the virus to cellular receptors, initiates infection, determines species specificity, and promotes viral particle internalization via caveolin-mediated endocytosis. Large envelope Protein, HBV-C (P.pastoris, 180a.a, His) is the recombinant Virus-derived Large envelope protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Large envelope Protein, HBV-C (P.pastoris, 180a.a, His) is 180 a.a., with molecular weight of 20.9 kDa.

Background

The Large Envelope Protein exists in two topological conformations, namely 'external' or Le-HBsAg, and 'internal' or Li-HBsAg. In its external conformation, the protein acts as a critical agent, binding the virus to cell receptors and initiating infection. This pivotal interaction not only establishes species specificity and liver tropism but also triggers virion internalization, predominantly through caveolin-mediated endocytosis. The Large Envelope Protein further facilitates fusion between the virion membrane and the endosomal membrane. In its internal conformation, the protein plays a crucial role in virion morphogenesis, functioning akin to a matrix protein and mediating contact with the nucleocapsid. Simultaneously, the middle envelope protein plays a vital role in virion budding, inducing a nucleocapsid-independent process. This budding mechanism leads to the formation of subviral lipoprotein particles, with a diameter of 22 nm, devoid of a nucleocapsid.

Species

Virus

Source

P. pastoris

Tag

N-6*His

Accession

P31868 (G2-G181)

Gene ID

/

Molecular Construction
N-term
6*His
HBV-C L (G2-G181)
Accession # P31868
C-term
Synonyms
S; Large envelope protein; L glycoprotein; L-HBsAg; LHB; Large S protein; Large surface protein; Major surface antigen
Molecular Weight

20.9 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Large envelope Protein, HBV-C (P.pastoris, 180a.a, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Large envelope Protein, HBV-C (P.pastoris, 180a.a, His)
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HY-P702559
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