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  6. Latent TGF beta 1/TGFB1 Protein, Human (Biotinylated, HEK293, His-Avi)

Latent TGF beta 1/TGFB1 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78214
COA Handling Instructions

Latent TGF beta 1/TGFB1 Protein is a large extracellular matrix protein and an associated ligand of fibrillinmicrofibrils. Latent TGF beta 1/TGFB1 Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant Biotinylated protein (L30-S390, C33S) produced by HEK293 cells with His-Avi tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $260 In-stock
50 μg $570 In-stock
100 μg $910 In-stock
> 100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

Latent TGF beta 1/TGFB1 Protein is a large extracellular matrix protein and an associated ligand of fibrillinmicrofibrils. Latent TGF beta 1/TGFB1 Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant Biotinylated protein (L30-S390, C33S) produced by HEK293 cells with His-Avi tag[1][2].

Background

Latent TGF beta 1/TGFB1 Protein promotes fibrillin-1 and -2 assembly downstream of fibronectin in mouse embryonic fibroblasts[3].

In Vitro

LTBP-1 fragments (30 µg/mL) inhibit the incorporation of native LTBP-1 into fibroblast matrix[2].

Biological Activity

Immobilized Human ITGAV&ITGB6 His at 1 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1 His with the EC50 of ≤ 0.6 μg/mL determined by ELISA.

Species

Human

Source

HEK293

Tag

N-Avi;N-His

Accession

P01137 (L30-S390, C33S)

Gene ID
Molecular Construction
N-term
His-Avi
TGFB1 (L30-S390, C33S)
Accession # P01137
C-term
Synonyms
CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1
Molecular Weight

40-50&13-15 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

Latent TGF beta 1/TGFB1 Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Latent TGF beta 1/TGFB1 Protein, Human (Biotinylated, HEK293, His-Avi)
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HY-P78214
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