Latent TGF beta 1/TGFB1 Protein, Human (Biotinylated, C33S, HEK293, His-Avi)

Latent TGF beta 1/TGFB1 Protein is a large extracellular matrix protein and an associated ligand of fibrillinmicrofibrils. Latent TGF beta 1/TGFB1 Protein, Human (Biotinylated, C33S, HEK293, His-Avi) is a recombinant Biotinylated protein (L30-S390, C33S) produced by HEK293 cells with His-Avi tag^[1][2].

Latent TGF beta 1/TGFB1 Protein promotes fibrillin-1 and -2 assembly downstream of fibronectin in mouse embryonic fibroblasts^[3].

LTBP-1 fragments (30 µg/mL) inhibit the incorporation of native LTBP-1 into fibroblast matrix^[2].

1.Immobilized Human ITGAV&ITGB6 His at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1 His with the EC₅₀ of ≤ 0.6 μg/mL determined by ELISA.
2.Immobilized Human ITGAV&ITGB6, His Tag at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC₅₀ of 30-65 ng/mL determined by ELISA.

Human

HEK293

N-Avi;N-8*His

P01137 (L30-S390, C33S)

7040  [NCBI]

Full Length of Mature Protein

Biotin

LSTSKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Approximately 13-15 kDa (Mature TGF beta 1) & 40-50 kDa (LAP), based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 90%, as determined by Bis-Tris PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. J Taipale, et al. Latent transforming growth factor-beta 1 associates to fibroblast extracellular matrix via latent TGF-beta binding protein. J Cell Biol. 1994 Jan;124(1-2):171-81.  [Content Brief]

  [2]. C Unsöld, et al. Latent TGF-beta binding protein LTBP-1 contains three potential extracellular matrix interacting domains. J Cell Sci. 2001 Jan;114(Pt 1):187-197.  [Content Brief]

  [3]. Matthias Przyklenk, et al. LTBP1 promotes fibrillin incorporation into the extracellular matrix. bioRxiv. 2022.

  [4]. Rui Cai, et al. LTBP1 promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fibroblasts transformation. J Transl Med. 2020 Mar 26;18(1):139.  [Content Brief]

  [5]. Xiaojun Fu, et al. LTBP1 plays a potential bridge between depressive disorder and glioblastoma. J Transl Med. 2020 Oct 15;18(1):391.  [Content Brief]