Leukotriene A4 Hydrolase/LTA4H Protein, Mouse (sf9, His)

The LTA4H protein is a bifunctional zinc metalloenzyme that acts as an epoxide hydrolase to convert leukotriene A4 (LTA4) into proinflammatory leukotriene B4 (LTB4).It also acts as an aminopeptidase, cleaving N-terminal arginine and fighting inflammation, particularly through Pro-Gly-Pro (PGP) cleavage.Leukotriene A4 Hydrolase/LTA4H Protein, Mouse (sf9, His) is the recombinant mouse-derived Leukotriene A4 Hydrolase/LTA4H protein, expressed by Sf9 insect cells , with C-His labeled tag.

The Leukotriene A4 Hydrolase/LTA4H protein is a bifunctional zinc metalloenzyme with both epoxide hydrolase (EH) and aminopeptidase activities. Serving as an epoxide hydrolase, it catalyzes the conversion of leukotriene A4 (LTA4) to the pro-inflammatory mediator leukotriene B4 (LTB4). Additionally, LTA4H exhibits aminopeptidase activity, displaying a high affinity for N-terminal arginines in various synthetic tripeptides. Beyond its pro-inflammatory EH role, the protein may counteract inflammation through its aminopeptidase activity, which cleaves the neutrophil attractant tripeptide Pro-Gly-Pro (PGP)—a bioactive collagen fragment generated by matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL). Moreover, LTA4H participates in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, contributing to the generation of lipid mediators with potent anti-inflammatory and pro-resolving actions. The multifaceted activities of LTA4H highlight its intricate involvement in the regulation of inflammatory processes and lipid mediator biosynthesis.

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (R-AMC). The specific activity is 32.7pmoles/min/μg.

Mouse

Sf9 insect cells

C-His

P24527 (M1-D611)

16993  [NCBI]

Full Length

MPEVADTCSLASPASVCRTQHLHLRCSVDFARRTLTGTAALTVQSQEENLRSLTLDTKDLTIEKVVINGQEVKYTLGESQGYKGSPMEISLPIALSKNQEIVIEISFETSPKSSALQWLTPEQTSGKQHPYLFSQCQAIHCRAILPCQDTPSVKLTYTAEVSVPKELVALMSAIRDGEAPDPEDPSRKIYRFNQRVPIPCYLIALVVGALESRQIGPRTLVWSEKEQVEKSANEFSETESMLKIAEDLGGPYVWGQYDLLVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEISHSWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFHALGGWGELQNTIKTFGESHPFTKLVVDLKDVDPDVAYSSIPYEKGFALLFYLEQLLGGPEVFLGFLKAYVKKFSYQSVTTDDWKSFLYSHFKDKVDLLNQVDWNTWLYAPGLPPVKPNYDVTLTNACIALSQRWVTAKEEDLSSFSIADLKDLSSHQLNEFLAQVLQKAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWEEAIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQAVHTYQEHKASMHPVTAMLVGRDLKVD

Approximately 62 kDa,based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 25 mM Tris, 50 mM NaCl, 50% Glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.