LIV-1/SLC39A6 Protein, Human (sf9, His)

LIV-1/SLC39A6 is a zinc influx transporter that regulates zinc homeostasis and induces epithelial-mesenchymal transition (EMT).Used in conjunction with SLC39A10, it can promote cellular zinc absorption and trigger EMT.LIV-1/SLC39A6 Protein, Human (sf9, His) is the recombinant human-derived LIV-1/SLC39A6 protein, expressed by sf9 insect cells , with C-6*His labeled tag.

LIV-1/SLC39A6, a zinc-influx transporter, intricately regulates zinc homeostasis and contributes to the induction of epithelial-to-mesenchymal transition (EMT). Functionally, when forming a heterodimer with SLC39A10, this complex mediates cellular zinc uptake, serving as a pivotal trigger for EMT. The SLC39A10-SLC39A6 heterodimer not only controls NCAM1 phosphorylation but also influences its integration into focal adhesion complexes during EMT. The zinc influx facilitated by this heterodimeric complex plays a crucial role in inactivating GSK3B, leading to nuclear accumulation of unphosphorylated SNAI1, which subsequently down-regulates adherence genes like CDH1, thereby promoting loss of cell adherence. Beyond its involvement in EMT, the SLC39A10-SLC39A6 heterodimer plays a fundamental role in initiating mitosis by importing zinc into cells, triggering a pathway that culminates in the onset of mitosis. Additionally, this transporter complex contributes to T-cell receptor signaling regulation and facilitates proper zinc influx for meiotic progression during the oocyte-to-egg transition.

Human

Sf9 insect cells

C-6*His

Q13433-1 (F29-W325)

25800  [NCBI]

Extracellular Domain

FPQTTEKISPNWESGINVDLAISTRQYHLQQLFYRYGENNSLSVEGFRKLLQNIGIDKIKRIHIHHDHDHHSDHEHHSDHERHSDHEHHSEHEHHSDHDHHSHHNHAASGKNKRKALCPDHDSDSSGKDPRNSQGKGAHRPEHASGRRNVKDSVSASEVTSTVYNTVSEGTHFLETIETPRPGKLFPKDVSSSTPPSVTSKSRVSRLAGRKTNESVSEPRKGFMYSRNTNENPQECFNASKLLTSHGMGIQVPLNATEFNYLCPAIINQIDARSCLIHTSEKKAEIPPKTYSLQIAW

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.