LIV-1/SLC39A6 Protein, Rat (Sf9, His)

The LIV-1/SLC39A6 protein is an important zinc influx transporter that maintains zinc balance and drives epithelial-to-mesenchymal transition (EMT). It cooperates with SLC39A10 to trigger EMT by promoting zinc uptake, regulating NCAM1 phosphorylation, and integrating it into focal adhesion complexes. LIV-1/SLC39A6 Protein, Rat (Sf9, His) is the recombinant rat-derived LIV-1/SLC39A6 protein, expressed by Sf9 , with C-His labeled tag. The total length of LIV-1/SLC39A6 Protein, Rat (Sf9, His) is 291 a.a., with molecular weight of 55-65 kDa.

The LIV-1/SLC39A6 protein is a zinc-influx transporter that plays a crucial role in maintaining zinc balance and promoting the transition of epithelial cells to mesenchymal cells. By forming a heterodimer with SLC39A10, it facilitates the uptake of zinc, triggering the process of epithelial-to-mesenchymal transition (EMT). This heterodimer also regulates the phosphorylation of NCAM1 and its integration into focal adhesion complexes during EMT. The influx of zinc inactivates GSK3B, leading to the down-regulation of adherence genes like CDH1, which results in the loss of cell adherence. Additionally, the SLC39A10-SLC39A6 heterodimer is vital for initiating mitosis by importing zinc into cells, initiating a pathway that leads to the onset of mitosis. It also plays a role in regulating T-cell receptor signaling by mediating zinc uptake in activated lymphocytes. Furthermore, it regulates the influx of zinc necessary for proper meiotic progression to metaphase II (MII), facilitating the transition from oocyte to egg.

Rat

Sf9 insect cells

C-His

Q4V887 (L21-W311)

291733  [NCBI]

Approximately 46 kDa. The reducing (R) protein migrat es as 46 kDa in SDS-PAGE may be due to glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.