LRP-1 Cluster II Protein, Human (HEK293, Fc)

LRP-1 Cluster II protein is involved in endocytosis and phagocytosis of apoptotic cells. The LRP-1 Cluster II protein also serves as a receptor for Pseudomonas aeruginosa exotoxin A during microbial infection. LRP-1 Cluster II Protein, Human (HEK293, Fc) is the recombinant human-derived LRP-1 Cluster II protein, expressed by HEK293, with C-hFc labeled tag.

LRP-1 Cluster II Protein emerges as a multifunctional endocytic receptor, playing pivotal roles in both endocytosis and the phagocytosis of apoptotic cells. Its significance extends to essential functions in early embryonic development and cellular lipid homeostasis. Additionally, LRP-1 Cluster II is actively involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the localized metabolism of complexes formed between plasminogen activators and their endogenous inhibitors. Acting as an LRPAP1 alpha-2-macroglobulin receptor, it further participates in various cellular events, including the endocytosis and subsequent spread of TAU/MAPT. Moreover, LRP-1 Cluster II may modulate critical cellular processes such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling, and neurotransmission. Notably, in microbial infection, the protein functions as a receptor for Pseudomonas aeruginosa exotoxin A, highlighting its diverse and intricate roles in cellular physiology and host-pathogen interactions.

Loaded Unconjugated Human PDGF-BB, His,Avitag on NTA Biosensor, can bind Human cluster II Protein, Fc Tag with an affinity constant of 7.58 nM as determined in BLI assay.

Human

HEK293

C-hFc

Q07954-1 (R786-L1165)

4035

Partial

RPPIFEIRMYDAQQQQVGTNKCRVNNGGCSSLCLATPGSRQCACAEDQVLDADGVTCLANPSYVPPPQCQPGEFACANSRCIQERWKCDGDNDCLDNSDEAPALCHQHTCPSDRFKCENNRCIPNRWLCDGDNDCGNSEDESNATCSARTCPPNQFSCASGRCIPISWTCDLDDDCGDRSDESASCAYPTCFPLTQFTCNNGRCININWRCDNDNDCGDNSDEAGCSHSCSSTQFKCNSGRCIPEHWTCDGDNDCGDYSDETHANCTNQATRPPGGCHTDEFQCRLDGLCIPLRWRCDGDTDCMDSSDEKSCEGVTHVCDPSVKFGCKDSARCISKAWVCDGDNDCEDNSDEENCESLACRPPSHPCANNTSVCLPPDKL

Approximately 75-105 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with 10% trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.