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  3. MERS-CoV Nucleoprotein/NP Protein (sf9, His)

MERS-CoV Nucleoprotein/NP Protein (sf9, His)

Cat. No.: HY-P74754
COA Handling Instructions

MERS-CoV Nucleoprotein/NP Protein is a phosphorylated basic protein and the second largest structural protein of MERS-CoV, containing 413 amino acid residues. The Nucleoprotein combines with the RNA genome to form a nucleocapsid, which is important in viral replication and assembly. In addition, Nucleoprotein plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. MERS-CoV Nucleoprotein/NP Protein (sf9, His) is the recombinant Virus-derived MERS-CoV Nucleoprotein/NP protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of MERS-CoV Nucleoprotein/NP Protein (sf9, His) is 413 a.a., with molecular weight of ~46.51 kDa.

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Description

MERS-CoV Nucleoprotein/NP Protein is a phosphorylated basic protein and the second largest structural protein of MERS-CoV, containing 413 amino acid residues. The Nucleoprotein combines with the RNA genome to form a nucleocapsid, which is important in viral replication and assembly. In addition, Nucleoprotein plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. MERS-CoV Nucleoprotein/NP Protein (sf9, His) is the recombinant Virus-derived MERS-CoV Nucleoprotein/NP protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of MERS-CoV Nucleoprotein/NP Protein (sf9, His) is 413 a.a., with molecular weight of ~46.51 kDa.

Background

MERS-CoV Nucleoprotein/NP is a phosphorylated basic protein and the second largest structural protein of MERS-CoV, containing 413 amino acid residues. The Nucleoprotein combines with the RNA genome to form a nucleocapsid, which is important in viral replication and assembly. Nucleoprotein binds to the SPRY domain of the tripartite motif protein 25 (TRIM25) E3 ubiquitin ligase, thereby interfering with the association between TRIM25 and retinoic acid-inducible gene I (RIG-I) and inhibiting TRIM25-mediated RIG-I ubiquitination and activation, thereby, Type I IFN production is suppressed by Nucleoprotein. It packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. In addition, Nucleoprotein plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication[1][2].

Species

Virus

Source

Sf9 insect cells

Tag

C-His

Accession

AFS88943 (M1-D413)

Gene ID

14254601  [NCBI]

Molecular Construction
N-term
MERS-CoV NP (M1-D413)
Accession # AFS88943
His
C-term
Synonyms
MERS-CoV coronavirus NP Protein; MERS-CoV coronavirus Nucleocapsid Protein
Molecular Weight

Approximately 46.51 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% gly, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

MERS-CoV Nucleoprotein/NP Protein (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
MERS-CoV Nucleoprotein/NP Protein (sf9, His)
Cat. No.:
HY-P74754
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