METAP2/Methionine aminopeptidase 2 Protein, Mouse (sf9, His)

METAP2, also known as methionine aminopeptidase 2, plays a critical role in protein synthesis by co-translationally removing N-terminal methionine from nascent proteins. This enzymatic activity is particularly pronounced when the second residue in the primary sequence is small and uncharged (including residues such as Ala, Cys, Gly, Pro, Ser, Thr, or Val). METAP2/Methionine aminopeptidase 2 Protein, Mouse (sf9, His) is the recombinant mouse-derived METAP2/Methionine aminopeptidase 2 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of METAP2/Methionine aminopeptidase 2 Protein, Mouse (sf9, His) is 477 a.a., with molecular weight of ~60 kDa.

METAP2, also known as Methionine Aminopeptidase 2, is a protein with a crucial role in protein synthesis regulation. It cotranslationally removes the N-terminal methionine from nascent proteins, particularly when the second residue in the primary sequence is small and uncharged, such as Met-Ala, Cys, Gly, Pro, Ser, Thr, or Val. This process is essential for proper protein maturation. Furthermore, METAP2 plays a key role in protecting the eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases like EIF2AK2/PKR and EIF2AK1/HCR. By preventing these inhibitory phosphorylation events, METAP2 contributes to the efficient initiation of translation, highlighting its significance in cellular protein synthesis.

Mouse

Sf9 insect cells

C-His

O08663 (A2-Y478)

56307  [NCBI]

Approximately 60 kDa

Greater than 88% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.