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METAP1/Methionine aminopeptidase 1 Protein, Human

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METAP1 (or methionine aminopeptidase 1) plays a crucial role in protein synthesis by co-translationally removing N-terminal methionine from nascent proteins.This enzymatic activity is particularly relevant when the second residue in the primary sequence is small and uncharged (including residues such as Ala, Cys, Gly, Pro, Ser, Thr or Val).METAP1/Methionine aminopeptidase 1 Protein, Human is the recombinant human-derived METAP1/Methionine aminopeptidase 1 protein, expressed by E.coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

METAP1 (or methionine aminopeptidase 1) plays a crucial role in protein synthesis by co-translationally removing N-terminal methionine from nascent proteins.This enzymatic activity is particularly relevant when the second residue in the primary sequence is small and uncharged (including residues such as Ala, Cys, Gly, Pro, Ser, Thr or Val).METAP1/Methionine aminopeptidase 1 Protein, Human is the recombinant human-derived METAP1/Methionine aminopeptidase 1 protein, expressed by E.coli , with tag free.

Background

METAP1, or Methionine Aminopeptidase 1, is a protein crucial for protein synthesis regulation. It cotranslationally eliminates the N-terminal methionine from nascent proteins, especially when the second residue in the primary sequence is a small and uncharged amino acid, such as Met-Ala, Cys, Gly, Pro, Ser, Thr, or Val. This activity is vital for proper maturation and function of proteins. Additionally, METAP1 plays a pivotal role in cellular processes by being required for normal progression through the cell cycle. Its involvement in the cell cycle underscores its significance in fundamental biological events, further emphasizing its role in maintaining cellular homeostasis and proper functioning.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P53582 (M1-F386)

  • Gene ID
    23173  [NCBI]
  • Molecular Construction
    • N-term
    • METAP1 (M1-F386)
      Accession # P53582
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    METAP1; Methionine Aminopeptidase 1; Methionyl Aminopeptidase 1; Peptidase M 1; KIAA0094; MetAP 1; MetAP1A; MAP 1; MAP1A; Peptidase M

  • AA Sequence

    MAAVETRVCETDGCSSEAKLQCPTCIKLGIQGSYFCSQECFKGSWATHKLLHKKAKDEKAKREVSSWTVEGDINTDPWAGYRYTGKLRPHYPLMPTRPVPSYIQRPDYADHPLGMSESEQALKGTSQIKLLSSEDIEGMRLVCRLAREVLDVAAGMIKPGVTTEEIDHAVHLACIARNCYPSPLNYYNFPKSCCTSVNEVICHGIPDRRPLQEGDIVNVDITLYRNGYHGDLNETFFVGEVDDGARKLVQTTYECLMQAIDAVKPGVRYRELGNIIQKHAQANGFSVVRSYCGHGIHKLFHTAPNVPHYAKNKAVGVMKSGHVFTIEPMICEGGWQDETWPDGWTAVTRDGKRSAQFEHTLLVTDTGCEILTRRLDSARPHFMSQF

  • Predicted Molecular Mass

    43.2 kDa

  • Molecular Weight

    Approximately 38-50 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Glycine, 10% sucrose, 10% glycerol, 0.02% Tween 80, pH 3.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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