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HLA-B Protein, Human (N-His)

Cat. No.: HY-P71699A
COA Handling Instructions

HLA-B Protein, a member of the major histocompatibility complex (MHC) class I family, is acknowledged. HLA-B Protein, Human (N-His) is the recombinant human-derived HLA-B protein, expressed by E. coli , with N-6*His labeled tag. The total length of HLA-B Protein, Human (N-His) is 284 a.a., with molecular weight of ~33.33 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

HLA-B Protein, a member of the major histocompatibility complex (MHC) class I family, is acknowledged. HLA-B Protein, Human (N-His) is the recombinant human-derived HLA-B protein, expressed by E. coli , with N-6*His labeled tag. The total length of HLA-B Protein, Human (N-His) is 284 a.a., with molecular weight of ~33.33 kDa.

Background

HLA-B belongs to the major histocompatibility complex (MHC) class I family.

Biological Activity

Data is not available.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

Q96DW9 (25G-308I)

Gene ID

/

Molecular Construction
N-term
6*His
HLA-B (25G-308I)
Accession # Q96DW9
C-term
Synonyms
HLA-B protein; MHC class I antigen; HLA-B
AA Sequence

GSHSMRYFYTAMSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQICKTNTQTYRESLRNLRGYYNQSEAGSHTLQRMYGCDVGPDGRLLRGHDQYAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAAREAEQLRAYLEGLCVEWLRRYLENGKETLQRADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTIPI

Molecular Weight

Approximately 33.33 kDa

Purity
  • Greater than 90% as determined by reducing SDS-PAGE
Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, 50% glycerin, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

HLA-B Protein, Human (N-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
HLA-B Protein, Human (N-His)
Cat. No.:
HY-P71699A
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