1. Recombinant Proteins
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Virus Neuraminidase
  5. NA/Neuraminidase Protein, Influenza A H5N1 (H255Y, HEK293)

NA/Neuraminidase Protein, Influenza A H5N1 (H255Y, HEK293)

Cat. No.: HY-P73239
COA Handling Instructions

Neuraminidase (NA) is described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. Whereby, NA facilitates viral invasion of the upper airways, facilitates virus release during virus budding, prevents self-aggregation and ensures the efficient spread of the progeny virus from cell to cell, and also plays a role in the determination of host range restriction on replication and virulence. NA/Neuraminidase Protein, Influenza A H5N1 (H255Y, HEK293) is the recombinant Virus-derived NA/Neuraminidase protein, expressed by HEK293 , with tag free. and H255Y mutation. The total length of NA/Neuraminidase Protein, Influenza A H5N1 (H255Y, HEK293) is 449 a.a., with molecular weight of ~49.2 kDa.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Neuraminidase (NA) is described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. Whereby, NA facilitates viral invasion of the upper airways, facilitates virus release during virus budding, prevents self-aggregation and ensures the efficient spread of the progeny virus from cell to cell, and also plays a role in the determination of host range restriction on replication and virulence. NA/Neuraminidase Protein, Influenza A H5N1 (H255Y, HEK293) is the recombinant Virus-derived NA/Neuraminidase protein, expressed by HEK293 , with tag free. and H255Y mutation. The total length of NA/Neuraminidase Protein, Influenza A H5N1 (H255Y, HEK293) is 449 a.a., with molecular weight of ~49.2 kDa.

Background

Neuraminidase (NA) is described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. NA may facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. NA catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates, cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release and additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell or the infection would be limited to one round of replication. NA also plays a role in the determination of host range restriction on replication and virulence[1][2].

Biological Activity

Measured by its ability to cleave a fluorogenic substrate, 2’-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37℃.

Species

Virus

Source

HEK293

Tag

Tag Free

Accession

ABU94738.1 (M1-K449, H255Y)

Gene ID

/

Molecular Construction
N-term
H5N1 NA (M1-K449, H255Y)
Accession # ABU94738.1
C-term
Synonyms
NA; Neuraminidase
Molecular Weight

Approximately 49.2 kDa

Purity

/

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, 0.6% Triton X-100, 7% Trehalose, 6% Mannitol, pH 7.4

Endotoxin Level

/

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

NA/Neuraminidase Protein, Influenza A H5N1 (H255Y, HEK293) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
NA/Neuraminidase Protein, Influenza A H5N1 (H255Y, HEK293)
Cat. No.:
HY-P73239
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