MME/Neprilysin Protein, Rhesus Macaque (HEK293, His)

MME is a cell-surface peptidase member of the M13 family of zinc peptidases, which also includes endothelin converting enzymes (ECE-1 and ECE-2), KELL and PEX. MME cleaves peptide bonds on the amino side of hydrophobic amino acids and is the key enzyme in processing of a variety of physiologically active peptides, such as GRP, neurotensin (NT), and vasoactive intestinal peptide (VIP). MME is downregulated in nearly 50% of primary and metastatic prostate cancers. MME/Neprilysin Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived Neprilysin/CD10 protein, expressed by HEK293 , with N-His labeled tag.

MME is a cell-surface peptidase member of the M13 family of zinc peptidases, which also includes endothelin converting enzymes (ECE-1 and ECE-2), KELL and PEX. MME cleaves peptide bonds on the amino side of hydrophobic amino acids and is the key enzyme in processing of a variety of physiologically active peptides, such as GRP, neurotensin (NT), and vasoactive intestinal peptide (VIP). MME is downregulated in nearly 50% of primary and metastatic prostate cancers, independently predicting an inferior prognosis. In addition to its downregulation by androgen withdrawal, MME expression is also downregulated by methylation, suggesting its tumor-suppressive effects. Indeed, MME expression reduces growth, motility, and survival of prostate cancer cells in cell culture. Consistent with these observations, replacements of MME inhibit tumorigenicity of prostate cancer cells in xenograft experiments^[1].

Measured by its ability to cleave a peptide substrate, Mca-(ala7,lys(dnp)9)-bradykinin. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read). The specific activity is 4008.09 pmoL/min/µg, as measured under the described conditions.

Rhesus Macaque

HEK293

N-His

F7H3Y6 (Y52-W750)

707667  [NCBI]

Partial

YDDGICKSSDCIKSAARLIQNMDATAEPCADFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQLQSNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW

Approximately 95 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.