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  3. NKp80/KLRF1 Protein, Cynomolgus (HEK293, Fc)

NKp80/KLRF1 Protein, Cynomolgus (HEK293, Fc)

Cat. No.: HY-P77453
COA Handling Instructions

NKp80/KLRF1 Protein plays a pivotal role in the natural killer (NK)-mediated cytolysis of PHA-induced lymphoblasts, highlighting its significance in immune response. As a homodimer, it structurally arranges in cells. Its involvement in NK-mediated cytolysis contributes to the immune system's ability to eliminate specific cell populations, enhancing cellular defense mechanisms. NKp80/KLRF1 Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived NKp80/KLRF1 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of NKp80/KLRF1 Protein, Cynomolgus (HEK293, Fc) is 166 a.a., with molecular weight of ~53-75 kDa.

For research use only. We do not sell to patients.

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Description

NKp80/KLRF1 Protein plays a pivotal role in the natural killer (NK)-mediated cytolysis of PHA-induced lymphoblasts, highlighting its significance in immune response. As a homodimer, it structurally arranges in cells. Its involvement in NK-mediated cytolysis contributes to the immune system's ability to eliminate specific cell populations, enhancing cellular defense mechanisms. NKp80/KLRF1 Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived NKp80/KLRF1 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of NKp80/KLRF1 Protein, Cynomolgus (HEK293, Fc) is 166 a.a., with molecular weight of ~53-75 kDa.

Background

NKp80/KLRF1 Protein is intricately linked to the natural killer (NK)-mediated cytolysis of PHA-induced lymphoblasts, playing a pivotal role in this immune response. The protein functions as a homodimer, highlighting its structural arrangement in the cellular context. Its involvement in NK-mediated cytolysis emphasizes its significance in the immune system's ability to target and eliminate specific cell populations, contributing to the intricate network of cellular defense mechanisms.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of U937 cells. When 5 x 104 cells/well are added to Recombinant Cynomolgus NKp80 coated plates (5 µg/mL, 100 µL/well), 67.36% cells will adhere after 1 hour incubation.

Species

Cynomolgus

Source

HEK293

Tag

N-hFc

Accession

Q8MI05 (V66-Y231)

Gene ID

102141941  [NCBI]

Molecular Construction
N-term
hFc
NKp80 (V66-Y231)
Accession # Q8MI05
C-term
Synonyms
Killer cell lectin-like receptor subfamily F member 1; Activating coreceptor NKp80; KLRF1; CLEC5C; ML
AA Sequence

VLLKCQKGSHSNTTEHEDIGDLKMNNGTRRNTSNKDLCVSRSADQTVLCQSEWLKYRGKCYWFSNEMKSWSDSYVYCLERKSHLLIIQDELEMAFIQKNLRQSNYVWMGLNFTSLKMTWTWVDGSPLDPKIFFIKGPAKENSCAAIKESKIYSETCSSVFKWICQY

Molecular Weight

Approximately 53-75 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, PH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

NKp80/KLRF1 Protein, Cynomolgus (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
NKp80/KLRF1 Protein, Cynomolgus (HEK293, Fc)
Cat. No.:
HY-P77453
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