Nogo Receptor/NgR Protein, Human (HEK293, His)

Nogo receptor/NgR protein is a multifunctional receptor for RTN4, OMG and MAG, mediating a variety of cellular responses. It also interacts with the sialylated gangliosides GT1b and GM1, chondroitin sulfate proteoglycans, and heparin. Nogo Receptor/NgR Protein, Human (HEK293, His) is the recombinant human-derived Nogo Receptor/NgR protein, expressed by HEK293 , with C-6*His labeled tag.

Nogo Receptor/NgR Protein acts as a versatile receptor for RTN4, OMG, and MAG, mediating diverse cellular responses. It also functions as a receptor for sialylated gangliosides GT1b and GM1 and chondroitin sulfate proteoglycans, while exhibiting the ability to bind heparin. Intracellular signaling is initiated through the coreceptor NGFR, leading to the activation of Rho and subsequent reorganization of the actin cytoskeleton. Nogo Receptor/NgR plays a pivotal role in axonal growth inhibition, influencing axon regeneration, and neuronal plasticity in the adult central nervous system . Additionally, it is essential for postnatal brain development, contributing to axon migration across the brain midline and corpus callosum formation. Acting in conjunction with RTN4 and LINGO1, it regulates neuronal precursor cell motility during cortical development. Furthermore, it participates in restricting the number of dendritic spines and synapses formed during brain development. Existing as a homodimer, Nogo Receptor/NgR interacts with various ligands, including MAG, RTN4, OMG, NGFR, and LINGO1, forming a complex network of molecular interactions that underlie its multifaceted roles in cellular processes and neural development.

Human Nogo Receptor protein immobilized on CM5 Chip can bind MAG/Siglec-4a with an affinity constant of 2.652 μM as determined in SPR assay.

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  Human Nogo Receptor protein immobilized on CM5 Chip can bind MAG/Siglec-4a with an affinity constant of 2.652 μM as determined in SPR assay.

Human

HEK293

C-6*His

Q9BZR6 (C27-S447)

65078  [NCBI]

Full Length of Mature Protein

CPGACVCYNEPKVTTSCPQQGLQAVPVGIPAASQRIFLHGNRISHVPAASFRACRNLTILWLHSNVLARIDAAAFTGLALLEQLDLSDNAQLRSVDPATFHGLGRLHTLHLDRCGLQELGPGLFRGLAALQYLYLQDNALQALPDDTFRDLGNLTHLFLHGNRISSVPERAFRGLHSLDRLLLHQNRVAHVHPHAFRDLGRLMTLYLFANNLSALPTEALAPLRALQYLRLNDNPWVCDCRARPLWAWLQKFRGSSSEVPCSLPQRLAGRDLKRLAANDLQGCAVATGPYHPIWTGRATDEEPLGLPKCCQPDAADKASVLEPGRPASAGNALKGRVPPGDSPPGNGSGPRHINDSPFGTLPGSAEPPLTAVRPEGSEPPGFPTSGPRRRPGCSRKNRTRSHCRLGQAGSGGGGTGDSEGS

60-90 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.

• [1]. /biology-dictionary/vero-cell.html