NRXN3/Neurexin-3 Protein, Human (HEK293, His)

Neurexin-3-beta is neuronal cell surface protein that is involved in cell recognition and cell adhesion. Neurexin-3-beta mediates intracellular signaling. NRXN3/Neurexin-3 Protein, Human (HEK293, His) is the recombinant human-derived NRXN3/Neurexin-3 protein, expressed by HEK293 , with C-His labeled tag.

NRXN3 encodes a member of a family of proteins that function in the nervous system as receptors and cell adhesion molecules. Extensive alternative splicing and the use of alternative promoters results in multiple transcript variants and protein isoforms for this gene, but the full-length nature of many of these variants has not been determined. Transcripts that initiate from an upstream promoter encode alpha isoforms, which contain epidermal growth factor-like (EGF-like) sequences and laminin G domains. Transcripts initiating from the downstream promoter encode beta isoforms, which lack EGF-like sequences. Genetic variation at this locus has been associated with a range of behavioral phenotypes, including alcohol dependence and autism spectrum disorder^{[1][2][3][4][5]}.

Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5×10⁴ cells/well are added to NRXN3 coated plates (0.8 μg/mL and 100 μL/well), approximately 46.72% will adhere specifically after 60 minutes at 37°C.

Human

HEK293

C-His

Q9HDB5-1 (S36-L357)

9369  [NCBI]

Partial

SSNVASSSSTSSSPGSHSQHEHHFHGSKHHSVPISIYRSPVSLRGGHAGATYIFGKSGGLILYTWPANDRPSTRSDRLAVGFSTTVKDGILVRIDSAPGLGDFLQLHIEQGKIGVVFNIGTVDISIKEERTPVNDGKYHVVRFTRNGGNATLQVDNWPVNEHYPTGRQLTIFNTQAQIAIGGKDKGRLFQGQLSGLYYDGLKVLNMAAENNPNIKINGSVRLVGEVPSILGTTQTTSMPPEMSTTVMETTTTMATTTTRKNRSTASIQPTSDDLVSSAECSSDDEDFVECEPSTGGELVIPLLVEDPLATPPIATRAPSITL

Approximately 50-70 kDa due to the glycosylation

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Güleç G, et al. Association of Nicotine Use Disorder with Neurexin 3 Gene Polymorphisms. Turk Psikiyatri Derg. 2021 Fall;32(3):160-166. English, Turkish.

  [2]. Liu L, et al. Circ_0001367 inhibits glioma proliferation, migration and invasion by sponging miR-431 and thus regulating NRXN3. Cell Death Dis. 2021 May 25;12(6):536.

  [3]. Restrepo S, et al. Modeling a Neurexin-3α Human Mutation in Mouse Neurons Identifies a Novel Role in the Regulation of Transsynaptic Signaling and Neurotransmitter Release at Excitatory Synapses. J Neurosci. 2019 Nov 13;39(46):9065-9082.

  [4]. Yang J, et al. The contribution of rare and common variants in 30 genes to risk nicotine dependence. Mol Psychiatry. 2015 Nov;20(11):1467-78.

  [5]. Zarrilli F, et al. Molecular analysis of cluster headache. Clin J Pain. 2015 Jan;31(1):52-7.