1. Recombinant Proteins
  2. Viral Proteins Others
  3. SARS-CoV-2 NSP16-NSP10 Protein

The multifunctional SARS-CoV-2 PP1ab protein is essential for viral RNA transcription and replication, utilizing proteases for multi-protein cleavage. It inhibits host translation by binding to the 40S subunit and blocks ribosomal mRNA entry channels, thereby hindering the antiviral response. NSP16/NSP10 Protein, SARS-CoV-2 is the recombinant virus-derived NSP16/NSP10, expressed by E. coli, with tag-free.

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Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Tamaño Precio Stock Cantidad
10 μg Obtener un presupuesto 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
50 μg Obtener un presupuesto 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
100 μg   Obtener un presupuesto  
Synthetic products have potential research and development risk.

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  • Actividad biológica

  • Technical Parameters

  • Properties

  • Descripciòn

Descripciòn

The multifunctional SARS-CoV-2 PP1ab protein is essential for viral RNA transcription and replication, utilizing proteases for multi-protein cleavage. It inhibits host translation by binding to the 40S subunit and blocks ribosomal mRNA entry channels, thereby hindering the antiviral response. NSP16/NSP10 Protein, SARS-CoV-2 is the recombinant virus-derived NSP16/NSP10, expressed by E. coli, with tag-free.

Species

Virus

Source

E. coli

Tag

Tag Free

Accession

P0DTD1-1 (S6799-N7096&A4254-Q4392)

Gene ID

43740578

Synonyms
Replicase polyprotein 1ab; ORF1ab polyprotein; rep; Severe acute respiratory syndrome coronavirus 2; 2019-nCoV; pp1ab; Endonuclease; Exonuclease; Helicase; Hydrolase; Lyase; Methyltransferase; Nuclease Nucleotidyltransferase; Protease; RNA-binding; RNA-directed RNA polymerase; Thiol protease; Transferase;
Predicted Molecular Mass
33.5 kDa & 14.8 kDa
Pureza

≥ 80%, as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl (pH 8.0), 500 mM NaCl, 5% glycerol, 2 mM MgSO4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Envío

Shipping with dry ice.

Documentación

SARS-CoV-2 NSP16-NSP10 Protein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Inquiry Information

Nombre del producto:
SARS-CoV-2 NSP16-NSP10 Protein
Cat. No.:
HY-P703532
Cantidad:
MCE Japan Authorized Agent: