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  4. NT5C3A/NT5C3 Protein, Human

NT5C3A/NT5C3 Protein, Human

Cat. No.: HY-P77455
Handling Instructions

NT5C3A/NT5C3 Protein, a nucleotidase, preferentially hydrolyzes cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP), emphasizing a potential role in cellular processes, with a preference for CMP. NT5C3A/NT5C3 Protein, Human is the recombinant human-derived NT5C3A/NT5C3 protein, expressed by E. coli , with tag free. The total length of NT5C3A/NT5C3 Protein, Human is 286 a.a., with molecular weight of ~32 kDa.

For research use only. We do not sell to patients.

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Description

NT5C3A/NT5C3 Protein, a nucleotidase, preferentially hydrolyzes cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP), emphasizing a potential role in cellular processes, with a preference for CMP. NT5C3A/NT5C3 Protein, Human is the recombinant human-derived NT5C3A/NT5C3 protein, expressed by E. coli , with tag free. The total length of NT5C3A/NT5C3 Protein, Human is 286 a.a., with molecular weight of ~32 kDa.
Background

NT5C3A/NT5C3 Protein, a nucleotidase, exhibits specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP). While it displays activity towards both substrates, CMP appears to be the preferred substrate for this enzyme. These catalytic preferences suggest the protein's involvement in the hydrolysis of specific nucleotide compounds, with a potential emphasis on cytidine monophosphate in cellular processes.
Species

Human
Source

E. coli
Tag

Tag Free
Accession

Q9H0P0 (M12-L297)
Gene ID

51251  [NCBI]
Molecular Construction
N-term
NT5C3A (M12-L297)
Accession # Q9H0P0
C-term
Synonyms
Cytosolic 5'-nucleotidase 3A; NT5C3; P5N1; UMPH1
Molecular Weight

Approximately 32 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

NT5C3A/NT5C3 Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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