Nucleocapsid/NP Protein, H7N9 (R4NE06, sf9, His)

Nucleoprotein/NP is critical in the influenza virus life cycle, encapsulating the negative-strand viral RNA to form a ribonucleoprotein (RNP) template. The nuclear localization signal of NP guides RNP into the host cell nucleus through α/β import. Nucleocapsid/NP Protein, H7N9 (R4NE06, sf9, His) is the recombinant Virus-derived Nucleocapsid/NP protein, expressed by Sf9 insect cells , with C-His labeled tag.

Nucleoprotein (NP) plays a pivotal role in the influenza virus life cycle by encapsidating the negative-strand viral RNA, forming the ribonucleoprotein (RNP) complex that serves as a template for transcription and replication. Facilitating the initiation of the infectious cycle requires the active transport of the RNP into the host cell nucleus, a task accomplished by NP's nuclear localization signals through the cellular importin alpha/beta pathway. Later in infection, the nuclear export of RNPs involves interactions with viral proteins, including NEP and M1, possibly in association with host exportin-1/XPO1. Notably, NP homomultimerizes to create the nucleocapsid, and its dynamic interplay with M1, governed by acidification driven by the M2 protein, regulates the exposure of NP's nuclear localization signals, facilitating the targeted transport of the RNP to the nucleus. Furthermore, NP forms essential contacts with viral genomic RNA, relying on a combination of electrostatic interactions and planar interactions to mediate protein-RNA binding.

Virus

Sf9 insect cells

C-His

R4NE06 (M1-N498)

23104229  [NCBI]

Full Length

MASQGTKRSYEQMETGGERQNATEIRASVGRMVSGIGRFYIQMCTELKLSDNEGRLIQNSITIERMVLSAFDERRNRYLEEHPSAGKDPKKTGGPIYRRRDGKWVRELILYDKEEIRRIWRQANNGEDATAGLTHLMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGIGTMVMELIRMIKRGINDRNFWRGENGRRTRIAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGLAVASGYDFEREGYSLVGIDPFRLLQNSQVFSLIRPNENPAHKSQLVWMACHSAAFEDLRVSSFIRGTRMVPRGQLSTRGVQIASNENMEAMDSNTLELRSRYWAIRTRSGGNTNQQRASAGQVSVQPTFSVQRNLPFERATIMAAFTGNTEGRTSDMRTEIIRMMESARPEDVSFQGRGVFELSDEKATNPIVPSFDMNNEGSYFFGDNAEEYDN

Approximately 57 kDa,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.