1. Recombinant Proteins
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Virus Nucleoprotein
  5. Nucleocapsid/NP Protein, H7N9 (R4NE06, sf9, His)

Nucleocapsid/NP Protein, H7N9 (R4NE06, sf9, His)

Cat. No.: HY-P75886
Handling Instructions

Nucleoprotein/NP is critical in the influenza virus life cycle, encapsulating the negative-strand viral RNA to form a ribonucleoprotein (RNP) template. The nuclear localization signal of NP guides RNP into the host cell nucleus through α/β import. Nucleocapsid/NP Protein, H7N9 (R4NE06, sf9, His) is the recombinant Virus-derived Nucleocapsid/NP protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Nucleocapsid/NP Protein, H7N9 (R4NE06, sf9, His) is 498 a.a., with molecular weight of ~57 kDa.

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Description

Nucleoprotein/NP is critical in the influenza virus life cycle, encapsulating the negative-strand viral RNA to form a ribonucleoprotein (RNP) template. The nuclear localization signal of NP guides RNP into the host cell nucleus through α/β import. Nucleocapsid/NP Protein, H7N9 (R4NE06, sf9, His) is the recombinant Virus-derived Nucleocapsid/NP protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Nucleocapsid/NP Protein, H7N9 (R4NE06, sf9, His) is 498 a.a., with molecular weight of ~57 kDa.

Background

Nucleoprotein (NP) plays a pivotal role in the influenza virus life cycle by encapsidating the negative-strand viral RNA, forming the ribonucleoprotein (RNP) complex that serves as a template for transcription and replication. Facilitating the initiation of the infectious cycle requires the active transport of the RNP into the host cell nucleus, a task accomplished by NP's nuclear localization signals through the cellular importin alpha/beta pathway. Later in infection, the nuclear export of RNPs involves interactions with viral proteins, including NEP and M1, possibly in association with host exportin-1/XPO1. Notably, NP homomultimerizes to create the nucleocapsid, and its dynamic interplay with M1, governed by acidification driven by the M2 protein, regulates the exposure of NP's nuclear localization signals, facilitating the targeted transport of the RNP to the nucleus. Furthermore, NP forms essential contacts with viral genomic RNA, relying on a combination of electrostatic interactions and planar interactions to mediate protein-RNA binding.

Species

Virus

Source

Sf9 insect cells

Tag

C-His

Accession

R4NE06 (M1-N498)

Gene ID

23104229  [NCBI]

Molecular Construction
N-term
H7N9 NP (M1-N498)
Accession # R4NE06
His
C-term
Synonyms
Influenza A H7N9 (A/Shanghai/2/2013) Nucleocapsid / NP Protein (His)
Molecular Weight

Approximately 57 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Nucleocapsid/NP Protein, H7N9 (R4NE06, sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Nucleocapsid/NP Protein, H7N9 (R4NE06, sf9, His)
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HY-P75886
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