1. Recombinant Proteins
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Virus Nucleoprotein
  5. Nucleoprotein/NP Protein, H3N2 (EPI541652, sf9, His)

Nucleoprotein/NP Protein, H3N2 (EPI541652, sf9, His)

Cat. No.: HY-P74670
Handling Instructions

Influenza virus nucleoprotein (NP) is a structural protein that coats viral negative-strand RNA. NP proteins protect viral RNA from degradation by cellular enzymes during the viral life cycle, fit the helical structure of RNP, regulate transcription and replication of viral RNA templates in a histone-like manner, and induce immunosuppression during infection. Nucleoprotein/NP Protein, H3N2 (EPI541652, sf9, His) is the recombinant Virus-derived Nucleoprotein/NP protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Nucleoprotein/NP Protein, H3N2 (EPI541652, sf9, His) is 498 a.a., with molecular weight of ~57.3 kDa.

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Description

Influenza virus nucleoprotein (NP) is a structural protein that coats viral negative-strand RNA. NP proteins protect viral RNA from degradation by cellular enzymes during the viral life cycle, fit the helical structure of RNP, regulate transcription and replication of viral RNA templates in a histone-like manner, and induce immunosuppression during infection. Nucleoprotein/NP Protein, H3N2 (EPI541652, sf9, His) is the recombinant Virus-derived Nucleoprotein/NP protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Nucleoprotein/NP Protein, H3N2 (EPI541652, sf9, His) is 498 a.a., with molecular weight of ~57.3 kDa.

Background

Influenza virus nucleoprotein (NP) is a structural protein that coats viral negative-strand RNA. NP is one of the main determinants of species specificity. The extent to which NP genes can be reprogrammed to cross the species barrier and adapt to a new host by mutation. NP proteins protect viral RNA from degradation by cellular enzymes during the viral life cycle, fit the helical structure of RNP, regulate transcription and replication of viral RNA templates in a histone-like manner, and induce immunosuppression (inhibition of effector cytokine synthesis and FcR drug signaling) during infection. NP is a very promising target for future vaccine development[1][2].

Species

Virus

Source

Sf9 insect cells

Tag

C-His

Accession

EPI541652 (M1-N498)

Gene ID

/

Molecular Construction
N-term
Nucleoprotein (M1-N498)
Accession # EPI541652
His
C-term
Synonyms
Influenza A H3N2 (A/Switzerland/9715293/2013) Nucleoprotein / NP Protein (sf9, His)
Molecular Weight

Approximately 57.3 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Nucleoprotein/NP Protein, H3N2 (EPI541652, sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Nucleoprotein/NP Protein, H3N2 (EPI541652, sf9, His)
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HY-P74670
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