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  3. OSM Protein, Cynomolgus (HEK293, His)

OSM Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Oncostatin M/OSM protein, expressed by HEK293, with C-His tag.

For research use only. We do not sell to patients.

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Description

OSM Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Oncostatin M/OSM protein, expressed by HEK293, with C-His tag.

Biological Activity

Human LIF R, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus OSM, His Tag in SPR assay (Biacore T200). Test result was comparable to standard batch.

Species

Cynomolgus

Source

HEK293

Tag

C-8*His

Accession

XP_045219762 (A27-R221)

Gene ID

102128264

Protein Length

Partial

Synonyms
Oncostatin M; OSM
AA Sequence

AMGSCSKEYRMLLGQLQKQTDLMQDTSRLLDPYIRIQGLDIPKLREHCRESPGAFPSEETLRGLGRRGFLQTLNATLGRVLHRLADLEQHLPKAQDLERSGLNIEDLEKLQMARPNVLGLRNNVYCMAQLLDNSDMTEPTKAGRGTPQPPTPTPTSDVFQRKLEGCSFLRGYHRFMHSVGRIFSKWGESPNRSRR

Predicted Molecular Mass
23.3 kDa
Molecular Weight

Approximately 27-50 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Purity

≥ 95%, as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

OSM Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
OSM Protein, Cynomolgus (HEK293, His)
Cat. No.:
HY-P703730
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