PET hydrolase Protein, Thermobifida cellulosilytica (His, Strep)

PET hydrolase Protein, Thermobifida cellulosilytica (His, Strep) is the recombinant PET hydrolase, expressed by E. coli , with Strep, His labeled tag. ,

PET hydrolase is an enzyme that catalyzes the hydrolysis of cutin, a structural component of the plant cuticle, and exhibits esterase activity toward p-nitrophenol-linked aliphatic esters (pNP-aliphatic esters). This enzyme is capable of degrading the most abundant polyester plastic, poly(ethylene terephthalate) (PET), as well as the bioplastic poly(lactic acid) (PLLA). by similar: Its functionality also extends to the degradation of other polyester materials.

Others

E. coli

Strep;His

E9LVH8 (A2-F262)

/

Full Length

ANPYERGPNPTDALLEASSGPFSVSEENVSRLSASGFGGGTIYYPRENNTYGAVAISPGYTGTEASIAWLGERIASHGFVVITIDTITTLDQPDSRAEQLNAALNHMINRASSTVRSRIDSSRLAVMGHSMGGGGTLRLASQRPDLKAAIPLTPWHLNKNWSSVTVPTLIIGADLDTIAPVATHAKPFYNSLPSSISKAYLELDGATHFAPNIPNKIIGKYSVAWLKRFVDNDTRYTQFLCPGPRDGLFGEVEEYRSTCPF

Approximately 30 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, pH 8.0, 200 mM NaCl, 5% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. /biology-dictionary/hela-cell.html  [Content Brief]