2. Recombinant Proteins
  3. Enzymes & Regulators
  4. PIK3R3 Protein, Human (Sf9, His, GST)

PIK3R3 Protein, Human (Sf9, His, GST)

Cat. No.: HY-P701744
Handling Instructions

PIK3R3 protein, through its SH2 domain, interacts with phosphorylated protein-tyrosine kinases, crucially regulating their kinase activity. During insulin stimulation, PIK3R3 binds to IRS-1. Operating as a heterodimer with a p110 catalytic subunit, it interacts with AXL, expanding its functional network. PIK3R3 Protein, Human (Sf9, His, GST) is the recombinant human-derived PIK3R3 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of PIK3R3 Protein, Human (Sf9, His, GST) is 460 a.a., .

For research use only. We do not sell to patients.

Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

PIK3R3 protein, through its SH2 domain, interacts with phosphorylated protein-tyrosine kinases, crucially regulating their kinase activity. During insulin stimulation, PIK3R3 binds to IRS-1. Operating as a heterodimer with a p110 catalytic subunit, it interacts with AXL, expanding its functional network. PIK3R3 Protein, Human (Sf9, His, GST) is the recombinant human-derived PIK3R3 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of PIK3R3 Protein, Human (Sf9, His, GST) is 460 a.a., .
Background

The PIK3R3 protein engages with activated (phosphorylated) protein-tyrosine kinases via its SH2 domain, playing a pivotal role in modulating their kinase activity. Additionally, during insulin stimulation, PIK3R3 forms a binding association with IRS-1. This protein operates as a heterodimer, comprising a regulatory subunit (PIK3R3) and a p110 catalytic subunit (PIK3CA, PIK3CB, or PIK3CD). Furthermore, PIK3R3 demonstrates interaction with AXL, contributing to its functional network.
Species

Human
Source

Sf9 insect cells
Tag

N-8*His;N-GST
Accession

Q92569 (Y2-R461)
Gene ID

8503
Molecular Construction
N-term
8*His-GST
PIK3R3 (Y2-R461)
Accession # Q92569
C-term
Synonyms
PIK3R3; Phosphatidylinositol 3-kinase regulatory subunit gamma; PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma; p55PIK
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

PIK3R3 Protein, Human (Sf9, His, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
PIK3R3 Protein, Human (Sf9, His, GST)
Cat. No.:
HY-P701744
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.