Protein G, Staphylococcus aureus (His)

Protein G is a cell wall protein isolated from Gram-negative streptococci, which mainly binds to mammalian IgGs via its Fc fragment. Protein G, Staphylococcus aureus (His) is a recombinant Protein G expressed by E. coli and tagged with C-6*His^{[1][2][3][4][5]}.

Protein G, Staphylococcus aureus, is a repetitive surface receptor derived from group G streptococci and can bind broadly to mammalian IgG, including human IgG3. Protein G, Staphylococcus aureus has no sequence homology with protein A, and its IgG-binding domain is predominantly β-sheet, with a secondary structure significantly different from the α-helical protein A. Protein G, Staphylococcus aureus is a classic antibody affinity purification ligand. Protein G, Staphylococcus aureus can be recombined with proteins A and L to construct chimeric proteins, resulting in richer antibody-binding properties. Protein G, Staphylococcus aureus is frequently engineered as a fusion tag for various immunological and biotechnological research^{[1][2][3][4][5]}.

Measured by its binding ability in a functional ELISA. Immobilized Protein G at 2μg/mL (100μL/well) can bind Biotinylated Human IgG1. The ED₅₀ for this effect is 0.1099 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Protein G at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1. The ED₅₀ for this effect is 0.1099 μg/mL.

Staphylococcus aureus

E. coli

C-6*His

/

Approximately 27 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5 % trehalose, 5 % mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Ståhl S. The use of gene fusions to protein A and protein G in immunology and biotechnology. Pathol Biol (Paris). 1997 Jan;45(1):66-76.

  [2]. Ståhl S, et al. Engineered bacterial receptors in immunology. Curr Opin Immunol. 1993 Apr;5(2):272-7.

  [3]. Yonemoto K, et al. Redundant and Distinct Roles of Secreted Protein Eap and Cell Wall-Anchored Protein SasG in Biofilm Formation and Pathogenicity of Staphylococcus aureus. Infect Immun. 2019 Mar 25;87(4):e00894-18.

  [4]. Geoghegan JA, et al. Role of Surface Protein SasG in Biofilm Formation by Staphylococcus aureus. J Bacteriol. 2010 Nov 1;192(21):5628–5638.

  [5]. Roche FM, et al. The Staphylococcus aureus surface protein SasG and its homologues promote bacterial adherence to human desquamated nasal epithelial cells. Microbiology (Reading). 2003 Oct;149(Pt 10):2759-2767.