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  5. Ribonuclease J/RnJ Protein, Synechocystis sp. (His-SUMO)

Ribonuclease J/RnJ Protein, Synechocystis sp. (His-SUMO)

Cat. No.: HY-P71470
Handling Instructions

Ribonuclease J/RnJ Proteinas, an enzyme with 5'-3' exonuclease activity and potential endonuclease function, is crucial in ribosomal RNA (rRNA) maturation. It also plays a role in mRNA maturation/decay, contributing to intricate RNA processing, ensuring precise maturation, and quality control in the cellular environment. Ribonuclease J/RnJ Protein, Synechocystis sp. (His-SUMO) is the recombinant Ribonuclease J/RnJ protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Ribonuclease J/RnJ Protein, Synechocystis sp. (His-SUMO) is 640 a.a., with molecular weight of ~86.4 kDa.

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Description

Ribonuclease J/RnJ Proteinas, an enzyme with 5'-3' exonuclease activity and potential endonuclease function, is crucial in ribosomal RNA (rRNA) maturation. It also plays a role in mRNA maturation/decay, contributing to intricate RNA processing, ensuring precise maturation, and quality control in the cellular environment. Ribonuclease J/RnJ Protein, Synechocystis sp. (His-SUMO) is the recombinant Ribonuclease J/RnJ protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Ribonuclease J/RnJ Protein, Synechocystis sp. (His-SUMO) is 640 a.a., with molecular weight of ~86.4 kDa.
Background

Ribonuclease J (RnJ) is an enzyme exhibiting 5'-3' exonuclease activity, with a potential additional function as an endonuclease. It plays a crucial role in the maturation processes of ribosomal RNA (rRNA) and is implicated in mRNA maturation and/or decay in certain organisms. This multifunctional RNase is integral to the intricate processes of RNA processing, contributing to the precise maturation and quality control mechanisms within the cellular environment.
Species

Others
Source

E. coli
Tag

N-His;N-SUMO
Accession

P54123 (1M-640S)
Gene ID

23611654  [NCBI]
Molecular Construction
N-term
6*His-SUMO
RnJ (1M-640S)
Accession # P54123
C-term
Synonyms
rnj; slr0551; Ribonuclease J; RNase J
AA Sequence

MAKNTQTQALKILPLGGLHEIGKNTCVFEYDDEILLLDAGLAFPTDDMHGVNVVLPDMTYLRENREKIKGMVVTHGHEDHIGGIAYHLKQFDIPIIYGPRLAMALLRDKLEEAGMLERTNLQTVSPREMVRLGKSFVVEFIRNTHSIADSYCLAIHTPLGVVMHSGDFKIDHTPIDGEFFDLQKVAEYGEKGVLCLLSDSTNAEVPGITPSEASVIPNLDRVFSQAEGRLMVTTFASSVHRVNIILSLAQKHQRKVAVVGRSMLNVIAHARKLGYIKCPDNLFVPLKAARNLPDQQQLILTTGSQGEPLAAMTRISNGEHPQIKIRQGDTVVFSANPIPGNTIAVVNTIDRLMMQGANVIYGKHQGIHVSGHASQEEHKMLLALTRPKFFVPVHGEHRMLVKHSQMAQAQGIPSENIVIVNNGDVIELTGDRIRVAGQVPSGIELVDQAGIVHESTMAERQQMAEDGLVTVAAALSKTGTLLAYPEVHCRGVVMTIQPKLLEELIVRTIENFLTERWSEFTHGSNGSTEVSWNALQKELESSLQRLIKRELQSSPMVLLMLQTDTPIELDQVPQNVSTVSATSATPAPRKKVVLTKTPEPKVKAKPEKKVVTTAEPSAQPVSTTKVYRRSRKRSTTSVSS

Molecular Weight

Approximately 86.4 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Ribonuclease J/RnJ Protein, Synechocystis sp. (His-SUMO) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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