RNF216 Protein, Human (His)

The RNF216 protein accepts ubiquitin from specific enzymes and transfers it to the substrate, promoting its ubiquitination. It has antiviral activity, inhibits TNF- and IL-1-mediated NF-kappa-B activation, and promotes TNF- and RIP-mediated apoptosis. RNF216 Protein, Human (His) is the recombinant human-derived RNF216 protein, expressed by E. coli , with N-6*His labeled tag. The total length of RNF216 Protein, Human (His) is 865 a.a., .

RNF216, an E3 ubiquitin ligase, dynamically regulates diverse cellular processes by accepting ubiquitin from specific E2 ubiquitin-conjugating enzymes and transferring it to substrates to promote ubiquitination. Central to antiviral responses, RNF216 orchestrates the degradation of TRAF3, TLR4, and TLR9, consequently down-regulating NF-kappa-B and IRF3 activation along with beta interferon production. Additionally, it influences autophagy by ubiquitinating BECN1, leading to its degradation and subsequent inhibition of autophagy. In the realm of synaptic plasticity, RNF216 modulates ARC-dependent processes by mediating ARC ubiquitination, resulting in rapid proteasomal degradation. Essential for spermatogenesis and male fertility, RNF216 contributes to meiotic regulation by promoting the degradation of PRKACB through the ubiquitin-mediated lysosome pathway. Furthermore, it fine-tunes the gonadotropin-releasing hormone signal pathway by affecting the stability of STAU2, crucial for microtubule-dependent transport of neuronal RNA from the cell body to the dendrite. Inhibiting TNF and IL-1-mediated activation of NF-kappa-B, RNF216 also promotes TNF and RIP-mediated apoptosis, showcasing its multifaceted regulatory roles in cellular physiology.

Human

E. coli

N-6*His

Q9NWF9 (E2-F866)

54476

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.