SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, N-His)

Nucleoprotein/NP Protein is essential for viral replication and transcription. Nucleoprotein/NP Protein binds to viral RNA to form a ribonucleoprotein complex required for genome replication. Nucleoprotein/NP Protein promotes NLRP3 inflammasome activation to induce excessive inflammation. SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His) is the recombinant Virus-derived SARS-CoV-2 Nucleocapsid protein, expressed by E. coli , with N-His labeled tag.

Nucleoprotein/NP Protein is essential for viral replication and transcription. Nucleoprotein/NP Protein binds to viral RNA to form a ribonucleoprotein complex required for genome replication. Nucleoprotein/NP Protein induces strong antibody (Ab) and T cell responses, regulating innate and adaptive immunity^[1][3].
Nucleoprotein/NP Protein functions by secreting into the extracellular space, where it competes with host chemokines to bind to host glycosaminoglycans (GAGs)^[2].
Nucleoprotein/NP Protein induces excessive inflammation by promoting the activation of the NLRP3 inflammasome, which plays a role in COVID-related lung injury^[4].

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Nucleocapsid at 1μg/mL (100μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid antibody. The ED₅₀ for this effect is 2.637 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Nucleocapsid at 1 μg/mL (100μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid antibody. The ED₅₀ for this effect is 2.637 ng/mL.

Virus

E. coli

N-6*His

YP_009724397 (M1-A419, with mutations P13L, ERS31-33 deletion, R203K, G204R)

43740575

Full Length

MSDNGPQNQRNALRITFGGPSDSTGSNQNGGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSKRTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA

Approximately 48 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.