Serum Albumin/ALB Protein, Human (P.pastoris)

The serum albumin/ALB protein has multiple binding abilities and can bind water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin, and drugs. Its main effects include regulating blood colloid osmotic pressure to achieve homeostasis. Serum Albumin/ALB Protein, Human (P.pastoris) is the recombinant human-derived Serum Albumin/ALB protein, expressed by P. pastoris , with tag free.

The Serum Albumin/ALB protein serves a multifaceted role by binding to water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin, and drugs, indicative of its versatile binding capabilities. Its primary function likely revolves around the regulation of the colloidal osmotic pressure of blood, emphasizing its pivotal role in maintaining blood homeostasis. Notably, ALB acts as a major transporter for zinc, binding approximately 80% of all plasma zinc, and also serves as a significant transporter for calcium and magnesium, binding about 45% of circulating calcium and magnesium in plasma. The presence of potential calcium-binding sites suggests its involvement in calcium transport, with a distinct affinity rank order of zinc > calcium > magnesium. Additionally, ALB exhibits an intriguing interaction with the bacterial siderophore enterobactin, inhibiting enterobactin-mediated iron uptake of E. coli and potentially limiting the utilization of iron by enteric bacteria. Furthermore, ALB engages in interactions with FCGRT, regulating its homeostasis, and forms a covalently-linked complex with chromophore-bound alpha-1-microglobulin in plasma, highlighting its diverse roles in molecular interactions and maintaining its functionality in fatty acid binding.

Serum Albumin/ALB Protein, Human (5%, 1 week) improves the cell recovery rate, relative proliferation potential and proliferation capacity of spermatogonial stem cells (SSCs), reduces the apoptosis capacity of SSCs^[1].
Serum Albumin/ALB Protein, Human (1 mg/mL, 48 h) promotes the proliferation of human proximal tubular cells^[2].

Serum Albumin/ALB Protein, Human (5%, single transplantation with an amount of 0.7 × 10⁵ cells per testis) forms SSCs colonies after transplantation, reveals self-renewal and differentiation abilities in C57BL/6 mouse models^[1].

Human

P. pastoris

Tag Free

P02768-1 (D25-L609)

213  [NCBI]

Full Length of Mature Protein

DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

55-80 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jung SE, et al., Effect of serum replacement on murine spermatogonial stem cell cryopreservation. Theriogenology. 2021 Jan 1;159:165-175.  [Content Brief]

  [2]. Arici M, et al., Fatty acids carried on albumin modulate proximal tubular cell fibronectin production: a role for protein kinase C. Nephrol Dial Transplant. 2002 Oct;17(10):1751-7.  [Content Brief]